geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Separation of intact plasmalogens and all other phospholipids by a single run of high-performance liquid chromatography


, : Separation of intact plasmalogens and all other phospholipids by a single run of high-performance liquid chromatography. Analytical Biochemistry 370(1): 54-59

Plasmalogens are a unique subclass of glycerophospholipids characterized by the presence of a vinyl ether bond at the sn-I position of the glycerol backbone, and they are found in high concentration in cellular membranes of many mammalian tissues. However, separation of plasmalogens as intact phospholipids has not been reported. This article describes a high -performance liquid chromatographic method that can separate intact ethanolamine plasmalogens (pl-PEs) and choline plasmalogens (pl-PCs) as well as all other phospholipid classes usuallv found in mammalian tissues by a single chromatographic run. The separation was obtained using an HPLC diol column and a,gradient of a hexane/isopropanol/water system containing 1% acetic acid and 0.08% triethylamine. The HPLC method allowed a clear separation of plasmalogens from their diacyl analogues. The HPLC method, as applied to the study of peroxidation in human erythrocytes by a hydroperoxide, demonstrated that pl-PEs were targeted twice as much as their diacyl analogues.

US$19.90

PMID: 17599799

DOI: 10.1016/j.ab.2007.05.020


Other references

Gangwar, D.N.; Jain, I.S.; Gupta, A., 1981: Accidental freezing of the anterior segment by freon. Annals of Ophthalmology 13(12): 1347-1349

Vidimos, A.T.; Poblete-Lopez, C.; Gasbarre, C.C., 2011: Preface. Mohs Surgery. Dermatologic Clinics 29(2): Xiii-Xxiv

Marciniak E., 1973: The binding and activity of heparin in human plasma. Federation Proceedings 32(3 PART 1): 290

Filonov, A.M.; Piloyan, G.O., 1983: Study of the specific heat capacity Cp of ammonium nitrate in the temperature range 373-440 K with the aid of the DAK-1-1M differential automatic calorimeter. The specific heat capacity Cp of ammonium nitrate was measured in the temperature range 373-440 K. Two phase transitions were observed: transition of tetragonal NH4NO3 (phase II) into the cubic form (phase I), and fusion of cubic NH4NO3 at 442 K....

Moustafa, A.A.; Poletti, M., 2014: Neural and behavioral substrates of subtypes of Parkinson's disease. Parkinson's disease (PD) is a neurological disorder, associated with rigidity, bradykinesia, and resting tremor, among other motor symptoms. In addition, patients with PD also show cognitive and psychiatric dysfunction, including dementia, mi...

Tunkel, A.R., 2008: Adjunctive corticosteroids for adults in sub-Saharan Africa with bacterial meningitis. Current Infectious Disease Reports 10(4): 290-291

Ballestriero, R., 2011: Anatomical models and wax Venuses: art masterpieces or scientific craft works?. The art of wax modelling has an ancient origin but rose to prominence in 14th century Italy with the cult of votive artefacts. With the advent of Neoclassicism this art, now deemed repulsive, continued to survive in a scientific environment, where...

Takatani, T.; Morita, T.; Anami, A.; Akaeda, H.; Kamijo, Y.; Tsutsumi, K.; Noguchi, T., 1998: Appearance of Gymnodinium catenatum in association with the toxification of bivalves in Kamae, Oita Prefecture, Japan. In spring of 1996. three species of bivalves, scallop Chlamys nobilis, mussel Mytilus edulis, and short-necked clam Tapes japonica, were toxified in Kamae, Oita Prefecture, Japan. In this bay, the cultured scallop C. nobilis has been infested with...

Turkevich, E.A.; Petrov, A.S.; Vorob'ev, A.V., 1992: Morphology of renal tumors induced by ferric nitrilotriacetate in rats. Voprosy Onkologii (St Petersburg) 38(4-6): 753-755

Neviere, R.; Leroy, B.; Scherpereel, P.; Izard, D.; Hermant, I., 1989: Diagnosis of nosocomial pneumopathies: elastic fibers. Annales Francaises D'anesthesie et de Reanimation 8 Suppl: R261-R261