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The effect of cations on chymotrypsin from Aedes aegypti larvae

, : The effect of cations on chymotrypsin from Aedes aegypti larvae. Journal of Insect Physiology 18(4): 747-755

Several cations activated chymotrypsin from the larval mosquito, activation being proportional to the concentration of the salts. Ca2+ and Mg2+ were more effective than K+ and Na+. The pH optimum for the chymotrypsin was about 8·5 in the absence of cations, but greater than 10 in their presence. By dialysing the chymotrypsin preparation, about half the activity was lost but this was regained by adding Ca2+. Mosquito chymotrypsin appears to consist of at least two components, one being stable while, without cations, the other is unstable. Preliminary observations suggested that mosquito trypsin is inhibited by Ca2+.


PMID: 5016958

DOI: 10.1016/0022-1910(72)90200-4

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