+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Bactericidal and tumoricidal activities of synthetic peptides derived from granulysin

, : Bactericidal and tumoricidal activities of synthetic peptides derived from granulysin. Journal of Immunology 165(3): 1486-1490

Granulysin, a 9-kDa protein localized to human CTL and NK cell granules, is cytolytic against tumor cells and microbes. Molecular modeling predicts that granulysin is composed of five alpha-helices separated by short loop regions. In this report, synthetic peptides corresponding to the linear granulysin sequence were characterized for lytic activity. Peptides corresponding to the central region of granulysin lyse bacteria, human cells, and synthetic liposomes, while peptides corresponding to the amino or carboxyl regions are not lytic. Peptides corresponding to either helix 2 or helix 3 lyse bacteria, while lysis of human cells and liposomes is dependent on the helix 3 sequence. Peptides in which positively charged arginine residues are substituted with neutral glutamine exhibit reduced lysis of all three targets. While reduction of recombinant 9-kDa granulysin increases lysis of Jurkat cells, reduction of cysteine-containing granulysin peptides decreases lysis of Jurkat cells. In contrast, lysis of bacteria by recombinant granulysin or by cysteine-containing granulysin peptides is unaffected by reducing conditions. Jurkat cells transfected with either CrmA or Bcl-2 are protected from lysis by recombinant granulysin or the peptides. Differential activity of granulysin peptides against tumor cells and bacteria may be exploited to develop specific antibiotics without toxicity for mammalian cells.


PMID: 10903754

DOI: 10.4049/jimmunol.165.3.1486

Other references

Vasari Yrjo, 1997: Long-term environmental dynamics, a symposium in honor of the birthday of Herbert E. Wright, Jr., held September 1997 in Wengen in the Bernese Oberland, Switzerland. Geologi 49(9-10): 119-122

Abbasi, J., 2017: Large HIV Vaccine Trial Launches in South Africa. JAMA 317(4): 350-350

Teertstra David K.; Cerny Petr; Hawthorne Frank C.; Pier Julie; Wang L.M.n; Ewing Rodney C., 1998: Rubicline, a new feldspar from San Piero in Campo, Elba, Italy. The rubidium analogue of microcline, rubicline, (Rb,K)AlSi (sub 3) O (sub 8) , ideally RbAlSi (sub 3) O (sub 8) , was found in a pollucite-bearing rare-element pegmatite at San Piero in Campo, Elba, Italy. Rubicline is the first mineral with rubid...

Ribi, E.; Anacker, R.L.; Barclay, W.R.; Brehmer, W.; Middlebrook, G.; Milner, K.C.; Tarmina, D.F., 1968: Biology of the mycobacterioses. Structure and biological functions of mycobacteria. Annals of the New York Academy of Sciences 154(1): 41-57

Miriel, V.A.; Mauban, J.R.H.; Wier, W.G.; Blaustein, M.P., 1999: Heterogeneous i dynamics in resistance arteries at maintained diameter during myogenic and agonist-mediated contraction. Biophysical Journal 76(1 PART 2): A12, Jan

Ustimenko G.V.; Vasil'eva V.N., 1983: Correlations between productivity and photosynthetic parameters of introduced cowpea forms. After the long-term study of the reaction of cowpea forms of different origin on the climatic conditions (tropics of Cuba, subtropics of the USSR), the variability of photosynthetic parameters and of productivity were noted. Yield formation is a c...

Bunting, K.; Cooper, J.B.; Badasso, M.O.; Tickle, I.J.; Newton, M.; Wood, S.P.; Zhang, Y.; Young, D., 1998: Engineering a change in metal-ion specificity of the iron-dependent superoxide dismutase from Mycobacterium tuberculosis X-ray structure analysis of site-directed mutants. We have refined the X-ray structures of two site-directed mutants of the iron-dependent superoxide dismutase (SOD) from Mycobacterium tuberculosis. These mutations which affect residue 145 in the enzyme (H145Q and H145E) were designed to alter its...

Steele, C.W.; Karim, S.A.; Leach, J.D.G.; Bailey, P.; Upstill-Goddard, R.; Rishi, L.; Foth, M.; Bryson, S.; McDaid, K.; Wilson, Z.; Eberlein, C.; Candido, J.B.; Clarke, M.; Nixon, C.; Connelly, J.; Jamieson, N.; Carter, C.Ross.; Balkwill, F.; Chang, D.K.; Evans, T.R.Jeffry.; Strathdee, D.; Biankin, A.V.; Nibbs, R.J.B.; Barry, S.T.; Sansom, O.J.; Morton, J.P., 2016: CXCR2 Inhibition Profoundly Suppresses Metastases and Augments Immunotherapy in Pancreatic Ductal Adenocarcinoma. CXCR2 has been suggested to have both tumor-promoting and tumor-suppressive properties. Here we show that CXCR2 signaling is upregulated in human pancreatic cancer, predominantly in neutrophil/myeloid-derived suppressor cells, but rarely in tumor...

Ghani Q.P.; Enriquez B.; Hunt T.K.; Hussain M.Z., 1990: Prolyl hydroxylase activity in fibroblasts exposed to nad. FASEB Journal 4(7): A2120

Jaehne G.; Roesner M.; Winkler I.; Helsberg M., 1990: Synthesis and antiviral activity of n7 substituted acyclic purine nucleoside analogues first example of an antivirally active n7 isomer. Antiviral Research (SUPPL 1): 39