geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Does mammalian heart contain only the M2 muscarinic receptor subtype?


, : Does mammalian heart contain only the M2 muscarinic receptor subtype?. Life Sciences 60(13-14): 1023-1029

Five muscarinic acetylcholine receptor (mAChR) subtypes, m1–m5, have been cloned and sequenced to date. The question as to which mAChR subtypes exist in mammalian heart has been studied extensively and is still under considerable debate. We used the reverse transcriptase-polymerase chain reaction to amplify mRNA from adult rat ventricular myocytes, and found that these cells express mRNA for m1 and m2 mAChRs. Immunocytochemical analysis confirmed that m1 and m2, but not m3, mAChR proteins are present on the surface of these cells. Finally, the functional significance of these receptors was examined. Administration of the m1 mAChR antagonist pirenzepine inhibited the stimulatory effect of the muscarinic agonist carbachol on Ca2+ transients. These findings are consistent with the presence of at least two mAChR subtypes in mammalian heart, m1 and m2, and suggest that activation of m1 mAChRs is involved in the stimulatory effects of muscarinic agonists in mammalian heart.

US$19.90

PMID: 9121343

DOI: 10.1016/s0024-3205(97)00043-x


Other references

Obeid, J-Pierre.; Zafar, N.; E.H.kayem, J., 2016: Steroid Hormone Receptor Coregulators in Endocrine Cancers. Coregulators span a broad and extensive domain in modulating cellular transcriptional activity. Studies have established a dynamic role for such coregulators in various endocrine cancers. Steroid hormone receptors (SHRs) play a pivotal role in suc...

Gutierrez, J M., 2003: Myotoxic phospholipases. Toxicon. 15 December; 428: 825-986

Cascella, M., 2016: The controversial experiments on the intravenous administration of drugs (and air!) during the cholera epidemic of 1867 in Italy. Cholera ravaged many American and European cities in the nineteenth century. Likewise, Italy was struck by six epidemics since the morbus first appeared in 1835-1837. After the International Sanitary Conferences held in Paris in 1851, there was a...

Serban, A.S.rbu, M., 1981: Building solutions of training fields and horse races. Buletinul Institutuli Agronomic Cluj Napoca: ub 1981) 34119-122

Allman, R.M.; Goode, P.S.; Tamura, T.; Sauberlich, H.E.; Freeberg, L.E.; Thomas, D.R.; Burst, N.; Bartolucci, A.A., 1997: Nutrition-related laboratory tests and pressure ulcers. Journal of the American Geriatrics Society 45(9): S37

Monjuvent G.; Marnezy A., 1986: The process of evolution in drainage basins in the French Alps. Geologie Alpine 62: 87-105

Zhang YingJun; Jiang WenLan; F.Y.Kun; X.Z.en, 1999: Study on controlling shrub and weeds by sheep night penning: IV. Effects of fresh sheep dung and urine on mineral contents in soil and plant. The effects of fresh sheep dung and urine on changes in the chemical composition of soil and plants were studied at Guizhou Plateau Grassland Station, China in 1998. The results showed that the content of K in soil increased, and that K also affec...

Baba Y., 1984: Changes in erythrocytes of diabetic patients with reference to the pathogenesis of microangiopathy. In diabetic patients, there are microrheological changes in blood flow and in the erythrocytes themselves including intravascular red cell aggregation and a reduction in erythrocyte deformability. The erythrocyte surface electric charge (SEC), mem...

Kennett, M.J.; Hook, R.R.J., 2002: Tritrichomonas foetus: characterization of isolates and partial purification of a secreted cytotoxin. Putative virulence factors including extracellular proteases, hemagglutinin, hemolysins, and soluble cytotoxins may play significant roles in the pathogenesis of trichomoniasis. The cytotoxicity, hemagglutinating, and hemolytic activity of Tritric...

Della Rocca, G.J.; Leung, K.Sui.; Pape, H-Christoph., 2011: Periprosthetic fractures: epidemiology and future projections. Periprosthetic fractures are fractures that occur in association with an orthopaedic implant, most often used for joint arthroplasty or fracture fixation. They are associated with significant morbidity and increased mortality in some cases. The in...