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Dynamic interactions of hepatocytes with fibronectin substrata: temporal and spatial changes in the distribution of adhesive contacts fibronectin receptors, and actin filaments

, : Dynamic interactions of hepatocytes with fibronectin substrata: temporal and spatial changes in the distribution of adhesive contacts fibronectin receptors, and actin filaments. Experimental Cell Research 198(1): 179-182

We have studied the interactions, over several hours, of adult rat hepatocytes with fibronectin substrata in vitro by interference reflection microscopy and by laser scanning confocal immunomicroscopy using antisera against the fibronectin receptors, integrin alpha 5 beta 1 and nonintegrin Agp110, together with phalloidin, a specific marker for filamentous actin. Distinct alterations in the pattern of cell-substratum adhesive contacts, in the distribution of receptors, and in actin organization were observed with time in culture. Cells examined 2 to 3 h after inoculation had formed focal contacts at symmetrically distributed microextensions of the basal cell periphery that contained integrin alpha 5 beta 1, AGp110, and termini of actin filaments. On more prolonged incubation, over 5 to 6 h, increasing numbers of cells displayed a lamellar structure in close juxtaposition to the substratum circumscribing more than half of the basal cell surface and long filopodia emanating from the remaining part of the cell periphery. Integrin alpha 5 beta 1 and AGp110 were mainly concentrated at the inner and, to a lesser extent, outer boundaries of the lamellae, and actin filaments close to the basal surface overlayed the inner boundary of the adhesive lamella colocalizing with the receptors. Filopodial extensions contained neither receptor.


PMID: 1530751

DOI: 10.1016/0014-4827(92)90166-6

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Metz Harold L., 1968: Geology of the El Pilar fault zone, State of Sucre, Venezuela. Transactions of the Caribbean Geological Conference = Memorias - Conferencia Geologica del Caribe Pages 293-298

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Kao, K.J.; Pizzo, S.V.; McKee, P.A., 1980: Factor VIII/von Willebrand protein. Modification of its carbohydrate causes reduced binding to platelets. Journal of Biological Chemistry 255(21): 10134-9

Bidnenko, S.I.; Nazarchuk, L.V.; Fedorovskaya, E.A.; Lyutko, O.B.; Open'ko, L.B., 1992: Follow-up of antibacterial immunity in humans under altered radiation conditions. The comparative study of the isolation rate, level, antigenic and class specificity of serum antibodies to the causative agents of purulent septic infections (Staphyloccoccus aureus, Pseudomonas aeruginosa, Proteus mirabilis) and acute enteric inf...

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De-La-Cruz, M.; Serrano, E.; Montoro, V.; Marco, J.C.; Romeo, M.; Baselga, R.; Albizu, I.; Amorena, B., 1994: Subclinical mastitis in the Manchegan sheep: Relation with the number of somatic cells, incidence, etiology and production. A bacteriological study was conducted on the milk of 466 Manchegan ewes to determine the incidence of subclinical mastitis. The percentage of ewes with bacteriological isolation in either udder and the percentage of udders infected were found to b...

Gilmour, J.S.L., 1972: Thomas Johnson, botanical journeys in Kent & Hampstead. It could be argued that Thomas Johnson was the most discerning taxonomist of the early seventeenth century. His treatment of the difficult genus Ulmus for example in the second edition of Gerard's Herball has determined the whole of the subse...

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Gregg, J.; Irani, T., 2004: Use of information technology by County Extension agents of the Florida Cooperative Extension Service. Journal of extension 42(3)