geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Inhibition of CD4+ T cell activation and adhesion by peptides derived from the gp160


, : Inhibition of CD4+ T cell activation and adhesion by peptides derived from the gp160. Journal of Immunology 147(2): 475-482

It has been previously demonstrated that the HIV envelope glycoprotein gp160 can inhibit the activation of T cells triggered by phytohemagglutinin, anti-CD3 antibody and Ag, caused in part by the modulation of the expression of CD4. In this study, we show that gp160 is also able to inhibit the Ag-independent adhesion of CD4+ T cells to B cells as anti-CD4 antibodies do. In addition, synthetic peptides (14 to 21 mer) derived from the gp160 sequence and analogous to the putative binding site of gp160 to CD4 (residues 418-460), and also covering residues 460 to 474 inhibit the capacity of both CD4+ T cell proliferation induced by tuberculin and anti-CD3 antibody and adhesion. This was not associated with inhibition of Ca2+ flux in T cell activation. These inhibitory activities are specific because a) CD4+ T cells but not CD8+ T cells are susceptible to their effects, and b) soluble CD4 neutralizes the inhibitory activities. Peptides are, however, about 100- to 1000-fold less potent inhibitors than the native gp160. In addition, they do not induce CD4 modulation. It is thought therefore that at least part of the gp160 inhibitory activity is not secondary to CD4 modulation but may rely either upon steric hindrance of CD4-MHC class II interaction, of CD4/CD3 TCR complex interaction, or upon negative signaling through binding to CD4. The latter hypothesis is suggested by the inhibition by gp160, gp160-derived peptides, and anti-CD4 antibodies of the Ag-independent adhesion of CD4+ T cells. This adhesion process has been previously shown to be mediated by the LFA-1 and CD2 molecules and not by the TCR/CD3 complex and by CD4. Together, these results support the role of part of the 418-460 region of gp160 as a binding site to CD4, and suggest that binding of part of this region to CD4 can alter T cell proliferation and adhesion. It is proposed that these effects are mainly mediated by negative signaling through CD4.

US$29.90

PMID: 1677023


Other references

Guerrero, M.S.; Parsons, J.Thomas.; Bouton, A.H., 2012: Cas and NEDD9 Contribute to Tumor Progression through Dynamic Regulation of the Cytoskeleton. The Cas family proteins, p130(Cas) (Cas) and NEDD9, are adaptor molecules that regulate cytoskeletal dynamics to promote multiple cellular processes, including migration, invasion, proliferation, and survival. Because these functions are also crit...

True Frederick William, 1907: Observations on the type specimen of the fossil cetacean Anoplonassa forcipata Cope. Bulletin of the Museum of Comparative Zoology: rd University. Pages 97-106. 1907.

Moorman, J.W.; Melen, R.E.; Skillicorn, B.; Solomon, E.G., 1994: Three-dimensional endocardial mapping system using a novel x-ray imager and locating catheter. Percutaneous radiofrequency catheter ablation (RFCA) has a very high success rate for certain arrhythmias, such as Wolff-Parkinson-White syndrome and idiopathic ventricular tachycardias in the right ventricular outflow tract. These arrhythmias are...

de Andrade, J.Inês.Alves.; Ono, E.Akifumi.; de Menezes, G.Cruz.; Brasil, E.Martins.; Roubach, R.; Urbinati, E.Criscuolo.; Tavares-Dias, M.; Marcon, J.Luiz.; Affonso, E.Gusmão., 2006: Influence of diets supplemented with vitamins C and E on pirarucu (Arapaima gigas) blood parameters. This study evaluated the influence of diets supplemented with 500, 800, 1200 mg kg-1 of vitamin C (ascorbic acid or AA) and vitamin E (alpha-tocopherol or alpha-T) on the physiological responses of pirarucu fed for 2 months. Weight and mortality w...

Yadav, U.C.S.; Aguilera-Aguirre, L.; Ramana, K.V.; Boldogh, I.; Srivastava, S.K., 2011: Aldose reductase inhibition prevents metaplasia of airway epithelial cells. Goblet cell metaplasia that causes mucus hypersecretion and obstruction in the airway lumen could be life threatening in asthma and chronic obstructive pulmonary disease patients. Inflammatory cytokines such as IL-13 mediate the transformation of...

De-May, C.L.; Ali, A.B., 2013: Involvement of pre- and postsynaptic NMDA receptors at local circuit interneuron connections in rat neocortex. To investigate the involvement of N-Methyl-D-aspartate (NMDA) receptors in local neocortical synaptic transmission, dual whole-cell recordings - combined with biocytin labelling - were obtained from bitufted adapting, multipolar adapting or multip...

Schmidt, M.; Hyttel, P.; Avery, B.; Greve, T., 1995: Ultrastructure of in vitro matured bovine oocytes after controlled freezing in 10 percent glycerol. The purpose of this study was to investigate the developmental capacity of, and possible subcellular damage to, bovine oocytes upon freezing and thawing. Following in vitro maturation, bovine oocytes were dehydrated in 10% glycerol, frozen, thawed...

Abercrombie, J., 2016: Expert's comment concerning Grand Rounds case entitled "Diagnosis and treatment of a rectal-cutaneous fistula: a rare complication of coccygectomy" by Eyal Behrbalk, Ofir Uri, Charles Maxwell-Armstrong and Nasir A. Quraishi (Eur Spine J; DOI 10.1007/s00586-014-3579-1). European Spine Journal 25(6): 1923-1923

Rementzova, MM.; Korol, AG., 1974: Brucellosis in Muridae. Unknown

Chen, L.F.O.; Kuo, H.E.; Yang, S.F.; Chen, S.C.G., 1994: Differential amplification between root and leaf DNAs in soybean by RAPD markers. Of the 40 primers used, including sequencing primers, known gene sequences and arbitrary decamers, for RAPD (random amplified polymorphic DNA) analysis of DNA from 11 hydroponically grown varieties (2 local landraces, 2 cultivars, 2 pairs of isoge...