geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Protein-dependent conformational behavior of DNA in chromatin


, : Protein-dependent conformational behavior of DNA in chromatin. Biochemistry 26(10): 2878-2886

Information from circular dichroism (CD) and DNA thermal denaturation has been used in concert to study the conformational behavior of DNA in the extended 11-nm fiber of chromatin isolated from HeLa nuclei. This histone-dependent conformational states of the system were investigated by selectively removing the hydrophilic histone domains with trypsin. These were compared to acetylated chromatin from the same source. The integrated intensity of the positive CD band for DNA above 260 nm is found to increase with the content of relatively unstressed B-form DNA. This same increase is observed along the series of whole, H1-stripped, and trypsinized chromatin samples as protein is removed. Hence, the ration of percent hyperchromicity to integrated CD band intensity of the respective melting transitions provides useful information on the conformational stage of DNA in the three principal regions of the chromatin fiber: the central loop and flanking nucleosomal regions and the linker. Results from this study suggest that central loop DNA in both hyperacetylated and control chromatin relaxes as protein is removed. However, hyperacetylated chromatin shows significantly less dependence than control chromatin upon core histone hydrophilic domains in the flanking and linker regions. Thus, histone hyperacetylation evidently relaxes DNA in chromatin with no major overall conformational changes. A possible role of histone hyperacetylation may therefore be to reduce cooperativity in the unfolding transition in chromatin and thus provide for greater localized control of unfolding during transcription.

US$19.90

PMID: 3606996

DOI: 10.1021/bi00384a032


Other references

Kibar, M.; Toker, M.; Ozturk, S., 2005: Evaluation of echocardiographic examination findings in dogs with mitral valve prolapse. The present study was performed to investigate the prevalence of mitral valve prolapse (MVP) in dogs and to evaluate protrusive changes in the mitral valve by echocardiographic examination. 35 dogs in Turkey were examined, including clinically nor...

Nentwig, W., 1989: Seasonal and taxonomic aspects of the size of arthropods in the tropics and its possible influence on size-selectivity in the prey of a tropical spider community. During one year in Panama, the size (body length) of arthropods (representing the potential prey of a spider community) was determined by sampling with pitfall traps, bowl traps and sweep-netting. Most arthropods found were small and the distribut...

Fradkin, G.E.; Naumova, L.A.; Rainkova, E.V.; Aĭzenberg, A.O., 1979: Effect of the DNA and protein synthesis balance on the yield of gene mutations induced by gamma radiation of Escherichia coli cells. Radiobiologiia 19(5): 741-744

Peng, X.; Zhao, Y.; Li, X.; Wu, M.; Chai, W.; Sheng, L.; Wang, Y.; Dong, Q.; Jiang, H.; Cheng, B., 2016: Genomewide identification, classification and analysis of NAC type gene family in maize. NAC transcription factors comprise a large plant-specific gene family. Increasing evidence suggests that members of this family have diverse functions in plant growth and development. In this study, we performed a genomewide survey of NAC type gen...

Sigee, D.C.; Teper, J.; Levado, E., 1999: Elemental composition of the cyanobacterium Anabaena flos-aquae collected from different depths within a stratified lake. The elemental composition of cells of Anabaena flos-aquae was investigated by energy-dispersive electron probe X-ray microanalysis (10 kV) of mixed phytoplankton samples obtained from different depths within a stratified eutrophic freshwater lake....

Wang, M.; Sun, Z.; Huang, L., 2016: Advanced Research on MicroRNAs and EGFR-TKIs Secondary Resistance. 肺癌是癌症致死率最高的疾病,关于这个疾病的发生机制已得到部分阐明,其中表皮生长因子受体(epidermal growth factor receptor, EGFR)信号通路研究最为深入,在肺癌的发生中起着至关重要...

Bobbio Pallavicini E.; Porta C.; Fornasari P.M.; Viarengo G.; Ascari E., 1992: Thrombotic thrombocytopenic purpura ttp retrospective study of 84 patients and therapeutic prospects. Transfusion Science 13(1): 39-44

Anonymous, 2005: Working as a Home Office Inspector. The UK possesses a rigorous and comprehensive system of regulation of animal research, which many believe to be the best in the world. A key element of these regulations is the employment of a group of inspectors by the Home Office. Here, a Home O...

Milone, M.C.; Shaw, L.M., 2015: Breaking the therapeutic drug monitoring logistics barrier. Clinical Chemistry 60(12): 1471-1472

Woo, K.S.; Chook, P.; Raitakari, O.T.; Mcquillan, B.; Feng, J.Z.; Celermajer, D.S., 1998: Westernization of Chinese adults is associated with increased subclinical atherosclerosis. Circulation 98(17 SUPPL ): I581-I582, Oct 27