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Proteomics-based identification of outer-membrane proteins responsible for import of macromolecules in Sphingomonas sp. A1: alginate-binding flagellin on the cell surface


, : Proteomics-based identification of outer-membrane proteins responsible for import of macromolecules in Sphingomonas sp. A1: alginate-binding flagellin on the cell surface. Biochemistry 44(42): 13783-13794

A nonmotile Gram-negative bacterium, Sphingomonas sp. A], directly incorporates macromolecules such as alginate through a "super-channel" consisting of a pit formed on the cell surface, alginate-C, binding proteins in the periplasm, and an ATP-binding cassette transporter in the inner membrane. Here, we demonstrate the proteomics-based identification of cell-surface proteins involved in the formation of the pit and/or import of alginate. Cell-surface proteins were prepared from the outer membrane released as vesicles during the conversion of intact cells to spheroplasts. Seven proteins (p1-p7) with acidic isoelectric points were inducibly expressed in the outer membrane of strain A] cells grown on alginate and showed significant identity with bacterial cell-surface proteins (p1-p4,. TonB-dependent outermembrane transporter; p5 and p6, flagellin; and p7, lipoprotein). Each mutant with a disruption of the p1-p4 or p6 gene showed significant growth retardation in the alginate medium. Flagellin homologues (p5 and p6) were further analyzed because strain A1 forms no flagellum. p5 was found to be uniformly distributed on the cell surface by immunogold-labeling electron microscopy and to exhibit alginate binding with a nanomolar dissociation constant by a surface plasmon resonance sensor. The cell surface of the p6 gene disruptant differed from that of the wild-type strain A1 in that pit formation was incomplete and cell-surface structures shifted from pleats to networks. These results suggest that, distinct from bacterial flagellins constituting a helical filament of flagella, strain A1 cell-surface flagellin homologues function as receptors for alginate and/or regulators of cell-surface structures.

US$19.90

PMID: 16229468

DOI: 10.1021/bi050873b


Other references

Ninan, C.A.; Pankajakshan, A.S.; Radhakrishnan, V., 1963: Preliminary observations on the influence of pollen parent on copra content in coconuts (Cocos nucifera L.). Highly significant differences in the copra content of nuts of 2 Laccadive strains were obtained by using pollen of different varieties. Seven varieties were used as pollen parents in each case. Compared with nuts obtained by pollination with spic...

Jacques, P.F., 1999: The potential preventive effects of vitamins for cataract and age-related macular degeneration. Age-related cataract and age-related macular degeneration (AMD) are important public health problems. Approximately 50% of the 30 to 50 million cases of blindness worldwide result from unoperated cataract. In the US and other developed countries A...

Figueiredo-Fernandes, A.; Fontaínhas-Fernandes, A.; Monteiro, R.; Reis-Henriques, M.A.; Rocha, E., 2006: Effects of the fungicide mancozeb on liver structure of Nile tilapia, Oreochromis niloticus: assessment and quantification of induced cytological changes using qualitative histopathology and the stereological point-sampled intercept method. The effects of exposure to sublethal concentrations of mancozeb (0.5, 1.0, 2.5 and 5.0 mg/litre) for a short period (4 days) on histopathological changes in the liver of O. niloticus were studied, and the effectiveness of the stereological paramet...

Dubach, U.C., 1973: Kidney toxicity of analgesics. Deutsche Medizinische Wochenschrift 98(34): 1584-1584

Chuchalin, A.G., 2005: Dyspnea: pathophysiological and clinical aspects. Terapevticheskii Arkhiv 77(3): 5-14

Schaaf, J., 1941: Uber die Feststellung der Tollwut durch Laboratoriumsmethoden Tierarztl. Diagnosis of rabies by observation of Negri bodies was successful in 92% of all positive cases. Coloration was made following Gerlach (carbolic fuchsin - methylene blue). A new modification of the Gerlach stain for histological preparations is giv...

Mindlin, M.J.; Lang, N.; Maguire, H.; Walsh, B.; Verlander, N.Q.; Lane, C.; Taylor, C.; Bishop, L.A.; Crook, P.D., 2013: Outbreak investigation and case-control study: penta-resistant Salmonella Typhimurium DT104 associated with biltong in London in 2008. In August 2008 an outbreak of Salmonella Typhimurium DT104 occurred in South West London. Sixteen cases were identified with a particular multilocus variable number tandem repeat analysis (MLVA) pattern. In a matched case-control study 14 primary...

Parisot, J., 1951: World Health Organization and professional training in the field of medicine and public health. La Presse Medicale 59(33): 662-663

Fülöp, Béla.; Deak, Gábor.; Mencser, Zán.; Kuncz, Aám.; Barzó, Pál., 2009: Factors affecting the development of chronic hydrocephalus following subarachnoid hemorrhage, with special emphasis on the role of ventricular and lumbar drainage. Hydrocephalus is a common complication of aneurysmal subarachnoid hemorrhage. Numerous studies have dealt so far with the triggering cause of the chronic cerebrospinal fluid (CSF) absorptional and circulatory disorders. Despite the fact that these...

Tran, H.B.; Jersmann, H.; Truong, T.Thanh.; Hamon, R.; Roscioli, E.; Ween, M.; Pitman, M.R.; Pitson, S.M.; Hodge, G.; Reynolds, P.N.; Hodge, S., 2017: Disrupted epithelial/macrophage crosstalk via Spinster homologue 2-mediated S1P signaling may drive defective macrophage phagocytic function in COPD. We have previously established a link between impaired phagocytic capacity and deregulated S1P signaling in alveolar macrophages from COPD subjects. We hypothesize that this defect may include a disruption of epithelial-macrophage crosstalk via Sp...