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Use of chlortetracycline fluorescence for the detection of Ca storing intracellular vesicles in normal human erythrocytes

, : Use of chlortetracycline fluorescence for the detection of Ca storing intracellular vesicles in normal human erythrocytes. Journal of Cellular Physiology 143(2): 357-363

The uptake of chlortetracycline (CTC) and the nature of the fluorescence of CTC was studied in intact human erythrocytes from apparently healthy donors. The uptake of CTC at 22 degrees C proceeded with a t1/2 of about 3 min, and after 15 min a stable equilibrium was achieved with an intracellular accumulation by a factor of 5-6 relative to the medium concentration. The accumulation did not change in the range of CTC concentrations tested (20-500 microM).


PMID: 2332457

DOI: 10.1002/jcp.1041430221

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