+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Detection of the mycovirus OMSV in the edible mushroom, Pleurotus ostreatus, using an SPR biosensor chip

, : Detection of the mycovirus OMSV in the edible mushroom, Pleurotus ostreatus, using an SPR biosensor chip. Journal of Virological Methods 148(1-2): 120-124

A surface plasmon resonance (SPR) biosensor chip was developed for the rapid detection of the oyster mushroom spherical virus (OMSV), which causes a mushroom die-back disease, the symptoms of which include malformed fruiting bodies and retarded mycelial growth in the cultivated edible mushroom, Pleurotus ostreatus. An anti-OMSV monoclonal antibody (mAb) was generated initially using purified OMSV viral particles. For the fabrication of the biosensor chip, the anti-OMSV mAb was layered onto an activated carboxymethyl-dextran (CM-Dex) gold thin film. Analysis on the SPR angle shift showed that the bound mAb was 6.7 ng/mm(2) of the chip surface. Subsequently, the biosensor chip was applied to the detection of OMSV in the mushroom mycelial extract. It detected specifically OMSV in the extract in a concentration-dependent manner. Finally, the biosensor chip was employed for the detection of OMSV in the mushroom fruiting bodies collected from 10 commercial farms. Among the tested samples, OMSV was found to infect fruiting bodies from a farmland, and this was confirmed further via immunoblot analysis and a TAS-ELISA assay. In conclusion, the SPR biosensor chip combined with an anti-OMSV mAb evidenced superior performance, particularly with regard to the prompt detection of OMSV infection.


PMID: 18079008

DOI: 10.1016/j.jviromet.2007.10.025

Other references

Connolly, A.; Sampson, E.L.; Purandare, N., 2012: End-of-life care for people with dementia from ethnic minority groups: a systematic review. A systematic review of the literature was conducted to examine the relationship between ethnic minority status and provision of end-of-life care for people with dementia. It included all empirical research on people with dementia or severe cogniti...

Nogi, K.; Taniguchi, H.; Onoda, Y., 2002: Effects of the anti-ulcer agents ecabet sodium, cimetidine and sucralfate on acetylsalicylic acid-induced gastric mucosal damage deteriorated by renal failure in rats. Renal failure including post-renal transplantation increases the susceptibility of the upper gastrointestinal mucosa to injury. The aim of this study was to confirm the influence of renal failure on gastric mucosal barrier and the protective effec...

Pole,S.P.; Sudewad,S.M.; Kamble,S.K.; Borgaonkar,S.B., 2008: Heterosis for seed cotton yield and yield components in upland cotton. A study was made in upland cotton with 7x7 diallel to assess the extent of heterosis over mid parent, better parent and standard check (NHH 44) for seed cotton yield and its related attributes. The highest heterotic effects were recorded in the hy...

Nath, S.; Agholme, L.; Kurudenkandy, F.Roshan.; Granseth, Börn.; Marcusson, J.; Hallbeck, M., 2012: Spreading of neurodegenerative pathology via neuron-to-neuron transmission of β-amyloid. Alzheimer's disease (AD) is the major cause of dementia. During the development of AD, neurofibrillary tangles progress in a fixed pattern, starting in the transentorhinal cortex followed by the hippocampus and cortical areas. In contrast, th...

Perez Cabanas I.; D.M.guel Velasco M.; Rodriguez Garrido J.; Montes Diaz M.; Ortiz Hurtado H., 1991: Lower gastrointestinal hemorrhage due to a jejunal leiomyoblastoma. Revista Espanola de Enfermedades Digestivas 79(2): 147-151

Escudero A.; Gonzalez R.; Serra M.; Olmo J.; Gilabert S.; Aparisi L.; Wassel A.; Rodrigo J.M., 1991: Influence of anti hcv on survival of alcohol chronic liver disease acld analyzed with cox multiple regression model. Journal of Hepatology 13(SUPPL 2): S119

Nagy Bodor E.; Szuromi Korecz A., 2002: Results of sporomorph and ostracod studies of Quaternary sediments of Lake Balaton. This paper gives a survey of the results of the earlier studies made on ostromorphs and ostracods from shallow drillings in the bed of Lake Balaton, as well as some new results of recent research. Our studies suggest that water depth and water qua...

Yosida, T.H.; Taya, C., 1977: Studies on interspecific hybridization in the rodents part 1 artificial insemination between the norway rat female and black rat male and the resulting karyotypes in the hybrid blastocysts. Semen of the black rat [Rattus rattus] was inseminated artificially into the female Norway rat [R. norvegicus]. Of 20 rats, 9 became pregnant, 5 impregnated rats were dissected on 8-14 days after insemination and 1-4 embryos were found. No offspri...

West, B.; Cusser, A.; Etengoff, S.; Landsgaard, H.; LaBond, V., 2015: The use of FAST scan by paramedics in mass-casualty incidents: a simulation study. The Focused Abdominal Sonography in Trauma (FAST) scan is used to detect free fluid in the peritoneal cavity, or pericardium, to quickly assess for injuries needing immediate surgical intervention. Mass-casualty incidents (MCIs) are settings where...

Porterfield, J.D., 1975: JCAH policies stated: from the director to the surveyors. Hospital Medical Staff 4(8): 36-39