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Development of a molecular assay specific for the Leishmania donovani complex that discriminates L. donovanil/Leishmania infantum zymodemes: a useful tool for typing MON-1


, : Development of a molecular assay specific for the Leishmania donovani complex that discriminates L. donovanil/Leishmania infantum zymodemes: a useful tool for typing MON-1. Diagnostic Microbiology and Infectious Disease 60(1): 33-42

We have developed a simple, rapid, sensitive, and cost-effective typing method, based on the amplicon size of the K26 gene, capable of species/strain discrimination of Leishmania donovani complex strains causing visceral leishmaniasis (VL). It was evaluated on 112 strains and compared with multilocus enzyme electrophoresis (MLEE) typing. The K26 polymerase chain reaction (PCR) applied on 26 representative L. donovani complex strains gave 14 different amplicon sizes. The assay was specific to the L. donovani complex and discriminated L. infantum from L. donovani strains. MON-1 strains were also easily distinguished from other non-MON-1. Surprisingly, 29.3% of the Greek strains included in this study were MLEE typed as MON-98 and gave exclusively a 940-bp amplicon. The majority of Greek MON-1 strains gave also the 940-bp amplicon, whereas a 626-bp amplicon was consistently obtained with other European MON-1 strains. K26 PCR-restriction fragment length polymorphism, based on MON-1 K26 sequence polymorphism, gave 2 MON-1 subgroups. Application of the method may contribute to efficiently monitor VL.

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Other references

Sorokin, S.; Padykula, H.A.; Herman, E., 1959: Comparative histochemical patterns in developing mammalian lungs. Prenatal and postnatal lungs from 2 rodents with different gestations, albino rat (23 days) and guinea pig (68 days), were compared to determine the course of histochemical differentiation and its relation to birth. Common patterns exist, but diff...

Cresson, Charles, 1993: Well-trained climbers: ways to keep wayward vines in bounds. Horticulture 71: 8

Claire, V.O., 1927: Dwarf races of fishes under natural and artificial life conditions. Proceedings of the Congress Zool Anat Hist USSR: 2: 38-39

Ratjen, E., 1952: Simple method for determination of film focal distance. Nordisk Medicin 48(41): 1420-1421

García-Valdés, E.; Castillo, Mía.M.; Bennasar, A.; Guasp, C.; Cladera, A.M.; Bosch, R.; Engesser, K.H.; Lalucat, J., 2003: Polyphasic characterization of Pseudomonas stutzeri CLN100 which simultaneously degrades chloro- and methylaromatics: a new genomovar within the species. Strain CLN100 was isolated after enrichment on mineral medium with chloronaphthalene as the only carbon and energy source. It was able to use simultaneously and productively chloro- and methyl-derivatives of naphthalene and salicylate through a ch...

Garber S.S., 1987: Permeation pathway of potassium ions in single toxin modified sodium channels is asymmetric. Biophysical Journal 51(2 PART 2): 9A

van Rijt, S.H.; Bölükbas, D.A.; Argyo, C.; Wipplinger, K.; Naureen, M.; Datz, S.; Eickelberg, O.; Meiners, S.; Bein, T.; Schmid, O.; Stoeger, T., 2016: Applicability of avidin protein coated mesoporous silica nanoparticles as drug carriers in the lung. Mesoporous silica nanoparticles (MSNs) exhibit unique drug delivery properties and are thus considered as promising candidates for next generation nano-medicines. In particular, inhalation into the lungs represents a direct, non-invasive delivery...

Felner; Nowik, 1986: First-order valence phase transition in cubic YbxIn1-xCu2. Physical Review. B, Condensed Matter 33(1): 617-619

Zaremba, R.E.; Gebauer, S.B., 1994: Planning and management for the Karner blue butterfly in the Albany Pine Bush. Unknown

Ishizaka, K.; Dennis, E.G.; Hornbrook, M., 1964: Presence of reagin and 1A-globulin in saliva. Reaginic activity was detected in saliva from ragweed sensitive individuals. These specimens, as well as saliva from normal individuals, contained [gamma]1A-globulin and possibly a trace of albumin, but no other serum protein was detected.