+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Histone deimination as a response to inflammatory stimuli in neutrophils

, : Histone deimination as a response to inflammatory stimuli in neutrophils. Journal of Immunology 180(3): 1895-1902

Posttranslational modifications, such as the deimination of arginine to citrulline by peptidyl arginine deiminase (PAD4), change protein structure and function. For autoantigens, covalent modifications represent a mechanism to sidestep tolerance and stimulate autoimmunity. To examine conditions leading to histone deimination in neutrophils, we used Abs that detect citrullines in the N terminus of histone H3. Deimination was investigated in human neutrophils and HL-60 cells differentiated into granulocytes. We observed rapid and robust H3 deimination in HL-60 cells exposed to LPS, TNF, lipoteichoic acid, f-MLP, or hydrogen peroxide, which are stimuli that activate neutrophils. Importantly, we also observed H3 deimination in human neutrophils exposed to these stimuli. Citrullinated histones were identified as components of extracellular chromatin traps (NETs) produced by degranulating neutrophils. In contrast, apoptosis proceeded without detectable H3 deimination in HL-60 cells exposed to staurosporine or camptothecin. We conclude that histone deimination in neutrophils is induced in response to inflammatory stimuli and not by treatments that induce apoptosis. Our results further suggest that deiminated histone H3, a covalently modified form of a prominent nuclear autoantigen, is released to the extracellular space as part of the neutrophil response to infections. The possible association of a modified autoantigen with microbial components could, in predisposed individuals, increase the risk of autoimmunity.


PMID: 18209087

DOI: 10.4049/jimmunol.180.3.1895

Other references

Latifa G.A.; Ali S.; Bala N., 1986: Observations on the fecundity of silver jew johnius argentatus from the bay of bengal bangladesh. The fecundity of Johnius argentatus (Houttuyn, 1782) was estimated to be 109,848 to 1196,980 for a size range of 31.0 to 46.5 cm length. The relationships of fecundity to total length, body weight, gonad weight and average ova diameter were calcul...

Tan, R.J.; Miles, J.A., 1971: Further studies on feline respiratory virus diseases. 2. Immunodiffusion tests. New Zealand Veterinary Journal 19(1-2): 15-18

Barnes, D., 1999: Relationship between new and return consultations and workload. General practitioners (GPs) in Tayside, with higher weekly surgery workloads, tended to have a greater proportion of return consultations compared with GPs with smaller workloads. By reducing the number of return consultations, GPs could have a no...

Brady-West, D.C.; Richards, L.; Thame, J.; Moosdeen, F.; Nicholson, A., 1998: Cancrum oris (noma) in a patient with acute lymphoblastic leukaemia. A complication of chemotherapy induced neutropenia. Cancrum oris (noma) has been most commonly described in malnourished debilitated children with poor oral hygiene following systemic childhood infections such as measles, pertussis or scarlet fever. We describe a patient who developed this conditio...

Isaacs, A.J.; Drew, S.V.; McPherson, K., 2005: UK women doctors' use of hormone replacement therapy: 10-year follow up. To determine changes in the prevalence and duration of use of hormone replacement therapy (HRT) by women doctors over 10 years. Questionnaire survey of 1234 UK women doctors (randomized, stratified sample), compared with a similar survey in 1993....

Seguchi, M.; Matsuki, J., 1977: Studies on pancake baking part 3 effects of ph mixing time and moisture level on the quality of pancake from reconstituted flour. Cake [wheat] flour was fractionated into water-solubles (5.31%), gluten (5.38%), tailings (15.0%) and prime starch (73.0%) with acetic acid. Pan-cake with quality equal to that from parent flour was baked when the pH of batter using acid-fractiona...

Campbell, J.A., 1870: Note of a Case in which Severe Constitutional Effects Followed the Application of Cantharidine Blister. British Medical Journal 1(492): 569-569

Dimitrov E., 1976: Changes at the cell surface during in vivo and in vitro de differentiation in cellular metaplasia. Mueller Berat, N. Progress In Differentiation Research. Proceedings Of The Second International Conference. Copenhagen, Denmark, Sept. 8-12, . X 587p. Illus. North-Holland Publishing Company: Amsterdam, Netherlands, (dist. In Usa And Canada By Elsevier North-Holland, Inc. New York, N.y.). Isbn 0-7204-0580-7; Isbn 0-444-11200-6. 361-367

Arnold Chester Arthur, 1953: Some fossil woods from northern Alaska, 2 of Silicified plant remains from the Mesozoic and Tertiary of western North America. Papers of the Michigan Academy of Science, Arts and Letters 38: 9-20

Huang Cuiling; L.C.i, 2003: The effects of medicinal rhubarb (Rheum officinale) on the levels of 6 keto-prostaglandin F1 alpha (PGF1 alpha ) and thromboxane B2 (TXB2) in the renal cortex and urine of rats with diabetes. Diabetes was induced in Wistar rats by intraperitoneal injection of streptozotocin (STZ) 50 mg/kg. Rats showed typical symptoms of diabetes were randomly divided into 3 groups and 2 groups were treated with medicinal rhubarb (Rheum officinale) 1 g...