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Human Pcf11 enhances degradation of RNA polymerase II-associated nascent RNA and transcriptional termination


, : Human Pcf11 enhances degradation of RNA polymerase II-associated nascent RNA and transcriptional termination. Nucleic Acids Research 36(3): 905-914

The poly(A) (pA) signal possesses a dual function in 3' end processing of pre-mRNA and in transcriptional termination of RNA polymerase II (Pol II) for most eukaryotic protein-coding genes. A key protein factor in yeast and Drosophila Pol II transcriptional termination is the 3'-end processing factor, Pcf11. In vitro studies suggest that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Moreover, several mutant alleles of yeast Pcf11 effect termination in vivo. However, functions of human Pcf11 (hPcf11) in Pol II termination have not been explored. Here we show that depletion of hPcf11 from HeLa cells reduces termination efficiency. Furthermore, we provide evidence that hPcf11 is required for the efficient degradation of the 3' product of pA site cleavage. Finally, we show that these functions of hPcf11 require an intact pA signal.

US$19.90

PMID: 18086705

DOI: 10.1093/nar/gkm1112


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Feng, Q.L.; Pan, Z.S., 1991: Effects of red light and far-red light on Ca2+-ATPase activity in mung bean chloroplasts. Mung bean seedlings with 4 leaves were irradiated with red light (maximum wavelength 660 nm, intensity 4 micro mol/msuperscript 2 s) and far red light (730 nm, 2.77 micro mol/msuperscript 2 s) for 10 min, and their Ca2+-ATPase activity was determi...

Pruski, W., 1958: Observations on the effect of dam and sire on the quality of their progeny. Examples from Thoroughbred breeding are quoted to show that the sire rather than the dam passes on racing ability to the progeny, thus refuting the popular belief that the always exercises a greater influence on the progeny than the . It is emphas...

Anonymous, 1968: The Rhododendron and Camellia Year Book, 1969. No. 23. This issue of the Year Book contains much information on dwarf rhododendrons. T. H. Findlay and C. Ingram contribute articles on the subject, and E. H. M. and P. A. Cox describe the rhododendrons at Glendoick in Perthshire. Notes on rhododendrons...

Sarudny, N.A., 1910: Mitteilungen uber die Ornithologie von Turkestan. Messager Ornithologique Moskva, 1 99-117: 171-178

Knepper, L.; Biller, J.; Adams, H.P.; Yuh, W.; Ryals, T.; Godersky, J., 1990: MR imaging of basilar artery occlusion. We studied six patients with basilar artery occlusion using magnetic resonance imaging. Two patients also had arteriography. All had signs consistent with pontine infarction; three had a "locked in" syndrome. Magnetic resonance imaging (...

Anon., 1961: Sort and store: new method of handling Washington apples. Fruit delivered to the packing house is sized and graded immediately, and then stored in bins until it is required for packing and marketing. A small regular labour force can be used, but equipment is expensive, fruit handling is increased, and th...

Kumar, N.; Timmermans, C.; Das, M.; Pison, L.; Maessen, J.; Crijns, H., 2014: Re: CT imaging of complications of catheter ablation for atrial fibrillation. Clinical Radiology 69(8): E367-E368

Xu, Y.B.; Zheng, J.M.; Chen, H.G., 1992: Effects of liming and rhizobial inoculation on the growth of Leucaena leucocephala cv. Salvador in an acid soil. Techniques shown to improve growth of Leucaena leucocephala seedlings in acid soil (from China) under glasshouse conditions included inoculation with rhizobia and liming the soil or pelleting seeds with lime. On a neutral soil plant growth increas...

Kropf-Herwig, G.; Lau, S.; Weitzel, H.; Wahn, U., 1988: How many newborn infants have an increased risk of atopic disease?. Children of atopic parents as well as newborns with an increased cord blood IgE-level have a increased risk to develop atopic symptoms later in childhood under the influence of certain environmental factors. Out of 1203 newborns there were 3% with...

Hayakawa, T.; Horiuchi, S., 2003: From angstroms to micrometers: self-organized hierarchical structure within a polymer film. Angewandte Chemie 42(20): 2285-2289