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Sub-terminal sequences modulating IS30 transposition in vivo and in vitro

, : Sub-terminal sequences modulating IS30 transposition in vivo and in vitro. Journal of Molecular Biology 375(2): 337-352

Inverted repeats of insertion sequences (ISs) are indispensable for transposition. We demonstrate that sub-terminal sequences adjacent to the inverted repeats of IS30 are also required for optimal transposition activity. We have developed a cell-free recombination system and showed that the transposase catalyses formation of a figure-of-eight transposition intermediate, where a 2 bp long single strand bridge holds the inverted repeat sequences (IRs) together. This is the first demonstration of the figure-of-eight structure in a non-IS3 family element, suggesting that this mechanism is likely more widely adopted among IS families. We show that the absence of sub-terminal IS30 sequences negatively influences figure-of-eight production both in vivo and in vitro. These regions enhance IR-IR junction formation and IR-targeting events in vivo. Enhancer elements have been identified within 51 bp internal to IRL and 17 bp internal to IRR. In the right end, a decanucleotide, 5'-GAGATAATTG-3', is responsible for wild-type activity, while in the left end, a complex assembly of repetitive elements is required. Functioning of the 10 bp element in the right end is position-dependent and the repetitive elements in the left end act cooperatively and may influence bendability of the end. In vitro kinetic experiments suggest that the sub-terminal enhancers may, at least partly, be transposase-dependent. Such enhancers may reflect a subtle regulatory mechanism for IS30 transposition.


PMID: 18022196

DOI: 10.1016/j.jmb.2007.10.043

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Stephenson, L.S.; Latham, M.C.; Adams, E.J.; Kinoti, S.N.; Pertet, A., 1993: Physical fitness, growth and appetite of Kenyan school boys with hookworm, Trichuris trichiura and Ascaris lumbricoides infections are improved four months after a single dose of albendazole. We studied physical fitness with the Harvard Step Test, growth, and appetite in primary school boys infected with hookworm (96% baseline prevalence), Trichuris trichiura (98% prevalence) and Ascaris lumbricoides (41% prevalence) who received a sin...

Macior, LW., 1990: Pollination ecology of Pedicularis punctata Decne. (Scrophulariaceae) in the Kashmir Himalaya. The nectarless flowers of Pedicularis punctata were pollinated exclusively by Bombus foragers which collected pollen from the concealed anthers by high frequency wing muscle vibrations. The stigma contacted pollen in the bee's cervical area d...

Sharpe G.R.; Cox N.H., 1990: Unilateral nevoid basal cell carcinoma syndrome an individually controlled study of fibroblast sensitivity to radiation. A patient with unilateral nevoid basal-cell-carcinoma syndrome (NBCCS, Gorlin's syndrome) provided a unique opportunity to compare the radiation sensitivity of fibroblasts from the affected and unaffected sides of the same individual. Althoug...

Crowder, Larry B., 1984: Character displacement and habitat shift in a native cisco in southeastern Lake Michigan: evidence for competition?. Copeia(4): 8-83

Becker, V., 1956: Liver structure and blood circulation. Arztliche Wochenschrift 11(38): 829-835

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Lushchin, I.K., 1975: Role of the unity of indicators in evaluation of transient disorders of cerebrovascular circulation. Vrachebnoe Delo: 34-37

Gruppi, A.; Cerbán, F.M.; Vottero-Cima, E., 1997: Exoantigens from trypanosoma cruzi contain cruzipain. This paper shows that human antibodies specific for exoantigens of pI 4.5 (Eas 4.5), released by the blood forms of the parasite, obtained from chagasic patients sera by immunoabsorption react with cruzipain, the major cysteinyl proteinase of Tryp...

Andrews, T., C.; Fenton, T.; Toyosaki, N.; Glasser, S., P.; Young, P., M.; Maccallum, G.; Gibson, R., S.; Shook, T., L.; Stone, P., H., 1993: Subsets of ambulatory myocardial ischemia based on heart rate activity: Circadian distribution and response to anti-ischemic medication. Background. Identification of whether episodes of ambulatory ischemia are caused by increases in myocardial oxygen demand or to episodic coronary vasoconstriction in patients with stable coronary disease may be important to guide selection of opti...

Pezzella, M.; Petrilli, R.; Lacava, V.; Chircu, L.V.; Corradini, S.G.; Corongiu, M., 1981: Purification method and partial immunological characterization of HBe Ag. Bollettino Dell'istituto Sieroterapico Milanese 60(1): 9-14