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The investigation of Protein A and Salmonella antibody adsorption onto biosensor surfaces by atomic force microscopy

, : The investigation of Protein A and Salmonella antibody adsorption onto biosensor surfaces by atomic force microscopy. Biotechnology and Bioengineering 99(4): 949-959

The investigation of Protein A and antibody adsorption on surfaces in a biological environment is an important and fundamental step for increasing biosensor sensitivity and specificity. The atomic force microscope (AFM) is a powerful tool that is frequently used to characterize surfaces coated with a variety of molecules. We used AFM in conjunction with scanning electron microscopy to characterize the attachment of protein A and its subsequent binding to the antibody and Salmonella bacteria using a gold quartz crystal. The rms roughness of the base gold surface was determined to be approximately 1.30 nm. The average step height change between the solid gold and protein A layer was approximately 3.0 +/- 1.0 nm, while the average step height of the protein A with attached antibody was approximately 6.0 +/- 1.0 nm. We found that the antibodies did not completely cover the protein A layer, instead the attachment follows an island model. Salt crystals and water trapped under the protein A layer were also observed. The uneven adsorption of antibodies onto the biosensor surface might have led to a decrease in the sensitivity of the biosensor. The presence of salt crystals and water under the protein A layer may deteriorate the sensor specificity. in this report, we have discussed the application and characterization of protein A bound to antibodies which can be used to detect bacterial and viral pathogens.


PMID: 17879317

DOI: 10.1002/bit.21644

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