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A brain-specific protein from Octopus vulgaris, Lam

, : A brain-specific protein from Octopus vulgaris, Lam. Journal of Neurochemistry 29(2): 235-244

Several major brain-specific proteins were detected in cephalopods by elctrophoretic analysis of the soluble proteins extracted from the optic lobes and other organs of octopus [O. vulgaris] and by 2 dimensional fractionation of the soluble proteins from optic lobes and hepatopancreases of octopus and squid [Loligo vulgaris]. One of the brain-specific proteins from octopus, identified as 0-1, was purified by chromatography on DEAE-cellulose, Sephadex G-150 and DEAE-Sephadex. The protein appears pure on the basis of several physiochemical criteria. Amino acid analysis indicates a high content of glutamic and aspartic acids or their amides (or both) and the lack of tryptophan. A MW of 17,000 was calculated from sodium dodecyl sulfate-gel electrophoresis, gel filtration and ultracentrifugation analysis. Preparation of a specific rabbit antiserum against 0-1 allowed its determination by agar immunodiffusion and complement fixation techniques. The latter procedure showed that the protein is absent outside the nervous system, is present in a concentration of several mg/g wet weight in octopus brain and is widely distributed within the octopus CNS and peripheral nervous system and in several molluscan species. It is present in optic lobes of octopus at early stages of development.


PMID: 886329

DOI: 10.1111/j.1471-4159.1977.tb09614.x

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