geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Acid washing effect on elemental and isotopic composition of whole beach arthropods: Implications for food web studies using stable isotopes


, : Acid washing effect on elemental and isotopic composition of whole beach arthropods: Implications for food web studies using stable isotopes. Acta Oecologica 34(1): 0-96

Inorganic carbon removal through acidification is a common practice prior to isotopic analysis of macroinvertebrate samples. We have experimentally tested the effect of acidification on the elemental and isotopic composition of a range of beach arthropod species. Acidification resulted in a significant depletion of 7.7% and 1.2% on average for carbon and nitrogen, respectively, suggesting that acid washing affects body carbon compounds other than carbonates. With a few exceptions, 13C and 15N showed no changes following 1 N HCl attack. Based on those exceptions, our results show that only those samples with a high CaCO3 content result in impoverished 13C as a consequence of acidification. Those suspected to be carbonate-free are not significantly affected. Concerning 15N values, only high carbonate species were affected when treated with HCl. As a standard protocol, it is recommended to acidify only carbonate-rich samples prior to 13C analyses. When possible, muscle tissue samples should be used instead of the entire organism.

US$19.90

DOI: 10.1016/j.actao.2008.04.002


Other references

Shilov, Y.I.; Gruzdeva, E.A., 2002: Influence of female sex steroid hormones on functions of the periferal blood phagocytic cells. Doklady Akademii Nauk 384(4): 550-552, June

Wang Zipan; Deprez Patrick P., 2000: Study on ecological structures of coastal lakes in Antarctica continent. Coastal region on the Antarctic continent, where it is under the influences both of ocean and ice sheet, as well as frequent human activities, could be considered as a fragile zone in Antarctic ecological environment. There are many lakes in coast...

Ozawa, H.; Hara, Y., 1968: Pharmacological actions of 2-aminoethylisothiuronium (AET) derivatives. IV. Inhibitory activity of N',N"-substituted-2-aminoethyl-isothiuronium derivatives on enzymes. Yakugaku Zasshi 88(7): 933-936

Tomilin, V.V.; Bereznyĭ, V.I., 1981: Determination of the hazard status for life and health in certain methods of violent crime. Sudebno-Meditsinskaia Ekspertiza 24(4): 52-53

Nemoto, Y.; Kearns, B.G.; Wenk, M.R.; Chen, H.; Mori, K.; Alb, J.G.; D.C.milli, P.; Bankaitis, V.A., 2000: Functional characterization of a mammalian Sac1 and mutants exhibiting substrate-specific defects in phosphoinositide phosphatase activity. The Saccharomyces cerevisiae SAC1 gene was identified via independent analyses of mutations that modulate yeast actin function and alleviate the essential requirement for phosphatidylinositol transfer protein (Sec14p) activity in Golgi secretory f...

Hamada, T.; Sano, M., 1981: Management and outcome of pregnancy in diabetic patients. Acta Obstetrica et Gynaecologica Japonica 33(2): 309-312

Radeke H.H.; Meier B.; Topley N.; Floege J.; Habermehl G.G., 1989: Cytokine induced release of excited oxygen species in cultured human glomerular mesangial cells hmc. European Journal of Cell Biology Supplement (26): 54

Odore, R.; Badino, P.; Re, G.; Barbero, R.; Cuniberti, B.; D'Angelo, A.; Girardi, C.; Fraccaro, E.; Tarantola, M., 2011: Effects of housing and short-term transportation on hormone and lymphocyte receptor concentrations in beef cattle. The experiment was designed to evaluate the effects of housing system and short-term transportation on the pituitary and adrenal response and on blood progesterone concentrations of beef cattle. Since the use of steroid hormones in farm animals ha...

Prosser E.S.; Pruthi R.; Csernansky J.G., 1989: Differences in the time course of dopaminergic supersensitivity following chronic administration of haloperidol molindone or sulpiride. The onset and persistence of changes in 3H-spiroperidol binding to dopamine (DA) D2 receptors were examined in rat mesolimbic and striatal brain regions following daily administration of haloperidol, molindone, or sulpiride for 3, 7, 14, or 28 day...

Pogacnik, A.; Pohar Marinsek, Z.; U.K.asovec, M., 1991: Diagnosis of liver hydatid by aspiration biopsy. Hydatid cysts were detected in 5 of 212 patients tested by fine needle aspiration biopsy at the Ljubljana Onchological Institute over a 13-month period in 1988-1989. The diameter of the needles used was 0.6-0.7 mm and no intervention-related compl...