+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium

, : Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. Extremophiles 13(1): 31-37

All members of the genera Haloarcula and Halomicrobium whose names have been validly published were surveyed for 16S rRNA gene polymorphism, and the transcription of the genes from two species was investigated during growth at different NaCl concentrations. The species of Haloarcula and Halomicrobium harbour at least two different 16S rRNA gene copies, and 18 new sequences of 16S rRNA genes were obtained. The type I and type II 16S rRNA genes of Haloarcula are divergent at 4.8–5.6% of their nucleotide positions. The type III and type IV 16S rRNA genes from Halomicrobium mukohataei JCM 9738T are 9.0% divergent, which represents the highest intraspecific divergent 16S rRNA genes so far seen. Phylogenetic analysis based on 16S rRNA genes indicated that all type I 16S rRNA genes were clustered, and the same was true for the type II 16S rRNA genes of Haloarcula species. The two clusters, respectively generated from type I and type II 16S rRNA genes, were sharply separated and their divergences (4.8–5.6%) are in the range of various divergence usually found between genera in the order Halobacteriales (about 5–10%). Results from reverse transcription-PCR showed that the type I and type II copies of Har. amylolytica BD-3T and type III and type IV copies of Hmc. mukohataei JCM 9738T were all transcribed to 16S rRNA molecules under different salt concentrations (15–28% NaCl).


PMID: 18836684

DOI: 10.1007/s00792-008-0194-2

Other references

Santell, J.P.; Hicks, R.W., 2005: Medication errors involving geriatric patients. Joint Commission Journal on Quality and Patient Safety 31(4): 233-238

Gusmeri D.; Centemeri M.D., 1985: Neuroleptanalgesia as an anesthesiological technique in translumbar aortographies. The old technique of neuroleptoanalgesia was revised to meet the requirements of translumbar aortography. The procedure started, 30 minutes before radiology by premedication with atropine, fentanyl and dehydrobenzoperidol. Patients were first plac...

Middleton, L.; Franck, H., 1981: The contribution of evoked visual potentials in the diagnosis of disseminated sclerosis. Revue D'oto-Neuro-Ophtalmologie 53(1): 5-11

Forrester, J.C.; Zederfeldt, B.H.; Hayes, T.L.; Hunt, T.K., 1970: Tape-closed and sutured wounds: a comparison by tensiometry and scanning electron microscopy. British Journal of Surgery 57(10): 729-737

Lemov, R., 2011: X-rays of inner worlds: the mid-twentieth-century American projective test movement. This essay begins to tell the neglected history of the projective test movement in the U.S. behavioral sciences from approximately 1941 to 1968. This cross-disciplinary enterprise attempted to use projective techniques as "X-ray" machine...

Kirk, P.M., 1994: IMI descriptions of fungi and bacteria No. 1205: Scolecobasidium variabile. Mycopathologia 127(1): 54-54

Mostofi, F.K., 1956: A study of 2678 patients with initial carcinoma of the bladder. I. Survival rates. Journal of Urology 75(3): 480-491

Gaylord, M.; Kolb, T.; Wallin, K.; Wagner, M., 2007: Seasonal dynamics of tree growth, physiology, and resin defenses in a northern Arizona ponderosa pine forest. During periods of resource stress, such as drought, allocating limited photosynthate between growth and defense is a crucial component of tree survival. Our objectives were to describe the seasonal dynamics of physiology, growth, and resin defense...

Bogner, R.H.; Laporte, S.L.; Hartz, B.M.rie; Albanese, D.L.; Bradley, M., 1997: Experimental evidence for the development of a microviscous layer near the surface of dissolving polyethylene glycol. The microenviromnent near the surface of dissolving polyethylene glycol 1450 (PEG 1450) samples was probed using microviscosity sensitive fluorescent probes. The microviscosity of 0.5 mm thick layers of solution was monitored by exciting the fluor...

Morabito, A.; MacKinnon, E.; Alizai, N.; Asero, L.; Bianchi, A., 2000: The anterior mediastinal approach for management of tracheomalacia. Tracheomalacia occurs as a primary developmental defect or may be secondary to vascular compression. It is common in association with esophageal atresia and tracheoesophageal fistula. Collapse of the weak trachea is a cause of recurrent respirator...