geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Preliminary study on the cultural conditions for the cultivation of schistosomula cells of Schistosoma japonicum in vitro


, : Preliminary study on the cultural conditions for the cultivation of schistosomula cells of Schistosoma japonicum in vitro. Chinese Journal of Zoonoses ruary; 21(2): 159-163

To explore the excellent cultural conditions for the cultivation of schistosomula cells of Schistosoma japonicum in vitro, 5 growth-promoting factors that promoted the proliferation of cultured cells, including transferrin, nucleotide, basic fibroblast growth factor (b-FGF), progesterone and non-essential amino acid and vitamin combination, were selected as the investigation elements. Two dose levels were chosen for every factor with two levels of addition or without addition. 16 conditional media were based on the RPM1-1640 used as the basal culture medium, and compounded according to orthogonal design. The schistosomula cells of S. japonicum were cultivated in these 16 culture media, the growth and proliferation of the cultured cells were determined by dynamic observations and the cell viability was assayed by AKP staining, in which the results of AKP assessment were intuitionally analyzed. The experimental results showed that the cultured tissue cells in cultivation plate wells No. 2, 4, 9, 13, and 14 appeared to be obviously proliferated and possessed higher AKP values than other cells. By analyzing the AKP values among all the cultured cells, it was demonstrated that the best factor combination to promote the proliferation of cultured cells was C1B1A1, which was transferrin, nucleotide and b-FGF combination. It concludes that the transferrin, nucleotide and b-FGF combination can promote obviously the proliferation of cultured cells from schistosomula cells of S. japonicum .

US$29.90


Other references

Devillers Charles; Chaline Jean; Laurin Bernard, 1990: In support of evolutionary embryology. La Recherche 21(222): 802-809

Gulrajani, R., 2010: Do people with mild COPD benefit from early pulmonary rehabilitation programmes?. People with mild to moderate COPD usually receive information about their condition from GPs or practice nurses, while those with moderate to severe disease have access to pulmonary rehabilitation programmes. This article describes a pilot of a pr...

Gawkowski, D.; Chikindas, M.L., 2013: Non-dairy probiotic beverages: the next step into human health. Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit to the host. The two main genera of microorganisms indicated as sources of probiotic bacteria are Lactobacillus and Bifidobacterium. Historical...

Akimov G.A.; Medvedev V.I.; Stepovik N.V.; Bakharev V.D.; Kaurov O.A., 1982: Use of vasopressin in patients with cerebral athero sclerosis accompanied by asthenic syndrome. The memory neuromodulator arginine-vasopressin was used for the 1st time in 32 patients with cerebral atherosclerosis. The trial tested the effect of the hypophsis posterior lobe hormone vasopressin on the mnestic processes and the patient's...

Hengtrakool, C.; Pearson, G.J.; Wilson, M., 2006: Interaction between GIC and S. sanguis biofilms: antibacterial properties and changes of surface hardness. The aim of this study was to investigate the interaction of Streptococcus sanguis with two glass-ionomer formulations (GIC:A containing fluoride and GIC:B without fluoride) with particular reference to bacterial growth and changes in hardness of t...

Ustyugov D.L., 2006: Real-time hydrodynamic model of Yakovlev ironstone deposit; objectives and solutions. Main goal of researches is estimation of values of inflows of water in example of Yakovlev of an iron-ore deposit using a method of mathematical modeling. Usage of constantly working mathematical models allows reacting operatively to fast varying...

Gunther, N.; Solaiman, D.; Fett, W., 2007: Processes for the production of rhamnolipids. A process for producing rhamnolipids involving culturing Pseudomonas chlororaphis strain NRRL B-30761 in a first aqueous culture medium containing about 0.3% NH.sub.4H.sub.2PO.sub.4, about 0.2% K.sub.2HPO.sub.4, about 0.2% carbon source, about 0.5...

Mozzherin, D.J.; McConnell, M.; Fisher, P.A., 1999: Drosophila replication and repair proteins: proliferating cell nuclear antigen (PCNA). Proliferating cell nuclear antigen (PCNA), a protein intimately involved in both replication and repair, has been identified in eukaryotes at all levels of evolution. Is primary sequence, Drosophila melanogaster PCNA is 73% identical to mammalian...

Farlow, M.R.; Lahiri, D.K.; Brashear, A.; Cyrus, P.A.; Gulanski, B., 1998: Metrifonate in the symptomatic treatment of Alzheimers disease Influence of apolipoprotein E genotype. Neurology 50(4 SUPPL 4): A88, April

Wellmer, F.; Riechmann, Jé.L., 2010: Gene networks controlling the initiation of flower development. The onset of flower formation is a key regulatory event during the life cycle of angiosperm plants, which marks the beginning of the reproductive phase of development. It has been shown that floral initiation is under tight genetic control, and de...