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Blastogenetic action of trypsin and chymotrypsin in lymphocytic cultures for a short time in normal subjects


, : Blastogenetic action of trypsin and chymotrypsin in lymphocytic cultures for a short time in normal subjects. Boll Ist Sieroterap Milan 45(7/8): 398-406

Trypsin and chymotrypsin are able to induce a blast transformation in "in vitro" short term lymphocyte cultures set up with peripheral blood of normal human subjects. The blast -inducing activity of these enzymes brings about the appearance, in the culture, of highly immature elements, with morphological characteristics of the histolym-phoid and reticulo-endothelial series, indistinguishable from those obtained in different cultures employing phytohemagglutinin, a Phaseolus vulgaris extract, the most active among the lymphocyte transforming agents so far known. The blast inducing activity of the enzymes is very likely the consequence of an enzyme damage of the protein structures of the cell membrane.

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