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Chromatostrip analysis of fatty acid derivatives


, : Chromatostrip analysis of fatty acid derivatives. Jour Amer Oil Chem Soc 38(11): 609-614

Chromatostrips provide a rapid and convenient method of examination of samples by spot tests and by ascending or descending chromatography. Ascending chromatography for the examination of mixtures is carried out on 12 x 140-mm. glass strips coated with 5% starch-bonded silicic acid, while descending chromatography is done on 12 x 200-mm. strips. The 5% starch-bonded silicic acid coatings are resistant to normal handling, may be marked with a soft lead pencil, and may be stored indefinitely for reference. Three detection systems are employed: fluorescent minerals for conjugated unsaturates, fluorescein-bromine for unsaturates, and 2[image],7[image]-dichloro-fluorescein for all types of compounds. Positive tests result in characteristic spots when observed under normal or ultraviolet illumination. This permits the classification of components, after separation on the chromatostrips, into the groupings of conjugated un-saturated, unsaturated (or easily brominated), and saturated compounds.

US$19.90

DOI: 10.1007/bf02633370


Other references

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