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Complete hydrolysis of steroid conjugates and quantitative extraction of free steroids from urine

, : Complete hydrolysis of steroid conjugates and quantitative extraction of free steroids from urine. Canadian Journal of Biochemistry 45(2): 191-202

A simple procedure for the serial hydrolysis and extraction of 5-ml urine samples is described. The samples are incubated at pH 4.45 for 24 hours at 37[degree]C with high concentrations of [beta]-glucuronidase and sulfatases from Helix pomatia. The incubated samples are freeze-dried and the residual solids are repeatedly extracted with a total of 15 ml of chloroform-methanol (1:2, v/v). The overall efficiency is over 99%. A method for the systematic estimation of urinary steroids based on the combined thin-layer and gas-liquid chromatographic analysis of total extracts is outlined.


PMID: 6021176

DOI: 10.1139/o67-022

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Shiau, Y.Y.; Kwan, H.W.; Chang, C., 1989: Prevalence of temporomandibular disorder syndrome (TMD) in university students--a third year report of the epidemiological study in Taiwan. A total of 2033 university students (1161 boys, 872 girls, ages ranged from 17 to 32 years) were examined according to an examination form. Pain and functional disorders of the head and neck muscles and TM joints, as well as the dental occlusal co...

Nilo, R., 1960: Considerations on 130 cases of biliary surgery. Revista Brasileira de Medicina 17: 412-423

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