+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Distribution within the cells and some properties of water-soluble and lipid-bound catalase in the normal liver and in transplanted hepatoma

, : Distribution within the cells and some properties of water-soluble and lipid-bound catalase in the normal liver and in transplanted hepatoma. Vop Med Khim 12(3): 227-234

Intracellular localization of activities of the soluble (in aqueous solutions) and lipid-bound forms of catalase was studied in cells of liver and hepatoma 22a in mice. Total content of the enzyme in intracellular structures of liver decreased in the following order: mitochondria, microsomes, hyalo-plasma, nuclei, nembranes, ribosomes; in hepatoma cells: mitochondria, microsomes, nuclei, membranes, hyaloplasma and ribosomes. Both forms of catalase exhibit 3 pH - optima of activity. Sensitivity towards controlled heating and effect of temperature on the activity were studied for both forms of catalase in homogenates and intracellular structures in normal liver and in hepatoma. Increased sensitivity of lipin - bound catalase of hepatoma towards the effect of KCN was demonstrated. Preliminary data on separation of both forms of catalase from normal liver and hepatoma by means of DEAE-Sephadex A-50 are reported. Hypothesis on the existence of the isoenzymes of catalase discussed.


Other references

Singh, D.V.; Srivastava, G.C.; Abdin, M.Z., 1999: Effect of ascorbic acid and benzyl adenine on superoxide dismutase activity in senna (Cassia angustifolia valh.) leaves in relation to senescence and water stress. An experiment was conducted in pots with senna, an annual medicinal plant, to study the effects of benzyladenine (BA, as a foliar spray) and ascorbic acid (AA, as a soil application) on superoxide dismutase (SOD) activity, an antioxidant enzyme du...

Kaszab, Z., 1967: Recent occurrence of the przewalski-horse (Equus przewalskii Porjakoff) in Mongolia. Allattani Kozlemenyek, 54: 63-65

Krieger, J.P.; Welsch, C.; Giesen-Crouse, E.; Schmidt, M.; Imbs, J.L., 1987: Effects of conversion enzyme inhibitors on the synthesis of angiotensin II by the isolated rat kidney. On the isolated perfused rat kidney, angiotensin-converting-enzyme activity was evaluated by two approaches: one, biochemical, through the measurements of the enzymatic activity on renal homogenate, the other, pharmacological, through the vasocons...

Pauwels, P.J.; Van Assouw, H.P.; Peeters, L.; Moeremans, M.; Leysen, J.E., 1992: Chronic treatment with sabeluzole protects cultured rat brain neurons from the neurotoxic effects of excitatory amino acids. The neuroprotective properties of the cognitive enhancer sabeluzole were investigated in rat brain neuronal cultures derived from the hippocampal formation of 17-day-old rat embryos. Measurement of the neuronal cytoskeletal microtubule-associated...

Oler, A.; Singh, M.; Ural, S.H., 1999: Bilateral ovarian stromal hyperplasia concealing a nonhilar, pure stromal-Leydig cell tumor. A case report. BACKGROUND: Of ovarian stromal tumors containing Leydig cells, nonhilar, pure stromal-Leydig cell tumor is rare. CASE: An obese, diabetic, borderline hypertensive 41-year-old woman with a five-year history of oligomenorrhea and amenorrhea presente...

Heath, Acg, 1987: A review of the origins and zoogeography of tick-borne disease in New Zealand. Tuatara 29(1-2): 9

Faas, B.H.W.; Ligthart, P.C.; Lomas Francis, C.; Overbeeke, M.A.M.; Von Dem Borne, A.E.G.K.; Van Der Schoot, C.E., 1997: Involvement of Gly96 in the formation of the Rh26 epitope. BACKGROUND: The Rh system, a complex blood group system, comprises at least 45 antigens. Red cells expressing c usually express Rh26. Rare cells that are c+ Rh:-26 give variable reactions with anti-c and may have weak expression of f (ce). STUDY D...

Berry Henry N.I.V.; Osberg Philip H., 2000: The Megunticook Formation. Annual Meeting - New England Intercollegiate Geological Conference, Guidebook 92: 54-70

Ballow, M.; Donshik, P.C.; Mendelson, L., 1985: Complement proteins and C3 anaphylatoxin in the tears of patients with conjunctivitis. Previous studies in our laboratory have demonstrated pollen-specific IgG antibodies in the tears of patients with vernal conjunctivitis (VC) and elevated tear IgG levels in patients with contact lens-induced giant papillary conjunctivitis (GPC). T...

Palamarczyk, G.; Pachecka, J., 1968: Influence of cysteine and urea on the enzymatic properties of cytochrome b2. Bulletin de L'academie Polonaise des Sciences. Serie des Sciences Biologiques 16(2): 89-95