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Electrophoretic mobility of canine serum maltase

, : Electrophoretic mobility of canine serum maltase. American Journal of Veterinary Research 19(73): 943-944

Dog serums were subjected to filter paper electrophoresis. After migration of the proteins, the filter paper strips were cut into 1-cm segments. Proteins of corresponding segments of duplicate strips were eluted together in 8 ml of a maltose solution prepared in 0.02 [image] phosphate buffer solution (Na2HPO4/KH2PO4), pH 6.6. Increased reducing power of the solution after 3 hours incubation at 39oC demonstrated maltase activity. When the maltose concentration was 5.6 x 10-4 [image], maltase activity was found associated with the alpha-1 and alpha-2 globulins, predominantly the leading edge of the alpha-2 globulin. When a higher concentration of maltose, 5.6 x 10-3 [image], was used, maltase activity was completely blocked.


PMID: 13583387

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