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In vitro culture of mucosa

, : In vitro culture of mucosa. Journal of Dental Research 39: 67-75

The objective of the study was to determine whether or not normal growth and maturation patterns of masticatory mucosa, dental epithelium, and respiratory mucosa were reproduced in vitro. Explants were taken from 15 and 19 day rat fetuses and were cultivated in tissue culture. The masticatory mucosa was taken from the hard palate and crest of the dental ridge. The dental epithelium was taken from the tooth bud. The respiratory mucosa was taken from the floor of the nasal cavity. The medium consisted of rat serum, embryo extract, and chicken plasma. Thrombin in balanced salt solution was added to produce a clot. The explants were cultured for a maximum of 10 days. They were observed in the flask at 24 hour intervals and photographed. On the third, fourth, and seventh days some explants were subcultured. Specimens were taken at 2, 4, 7, 8, and 10 days. It was found that the tissues could be cultured as organs to grow normally.


PMID: 14434271

DOI: 10.1177/00220345600390012301

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