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Influence of DNA on growth and viability of transformable group H streptococci

, : Influence of DNA on growth and viability of transformable group H streptococci. Acta Biochimica Polonica 14(2): 277-291

The addition of transforming DNA to a highly competent culture of group-H streptococci leads to death of a part of the population, and probably an increase in generation time of the viable cells. This effect of DNA manifests itself at saturation concentrations for transformation (about 5 /ng/ml), and is accentuated by increasing DNA concentrations up to 50 iig/ml. The maximum observed lethal effect led to the death of at least 65% of the recipient cells during the early logarithmic growth phase. Several hrs incubation of the recipient population with transforming DNA under optimal culture conditions leads to a 10-15-fold decrease in the number of colony-forming units, relative to that of a non-treated control. DNase-treated DNA is completely without effect on cell growth. The influence of DNA on cell growth is not due to its transforming activity, since the effect is accentuated by partially inactivated (by ultraviolet irradiation) DNA; and since both homologous and heterologous DNA are almost equally effective. The influence of DNA is exerted only on competent cells (when DNA uptake occurs). The dependence of the lethal effect of DNA on the competence of recipient cells indicates that at least 71% of the cells were capable of taking up exogenous DNA in a highly competent recipient population.


PMID: 4166052

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