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Method for the destruction of microbial cells

, : Method for the destruction of microbial cells. Prikl Biokhim Mikrobiol 3(3): 347-351

A desintegrator was developed to grind microbial cells. The ground material cooled at [long dash]40[long dash]45[degree] C was squeezed (at a pressure of 250-300 atm) through a slit with a clearance of 0.3-0.5 mm. The cells were destroyed with ice crystals. The performance of the apparatus was evaluated by the amount of cells remaining intact as well as by the inhibition of active proteins and subcellular structures in the supernatant after centrifugation. The apparatus was tested in several laboratories. The results were positive.


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Rupp, T.L.; Wesensten, N.J.; Balkin, T.J., 2010: Sleep history affects task acquisition during subsequent sleep restriction and recovery. The aim of the present study was to examine if sleep amount prior to sleep restriction mediated subsequent task acquisition on serial addition/subtraction and reaction time (RT) sub-tasks of the Automated Neuropsychological Assessment Metric. Elev...

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Nistor, D.; Nistor, D., 1979: Influence of long term fertilization on some properties of the soil, erosion and maize yields on sloping eroded land. In field trials in 1970-8 at Perieni on (a) a moderately and (b) a heavily eroded medium leached chernozem with a 12-16% slope, 0-300 kg N + 0-100 kg P2O5 + 0-50 kg K2O/ha was incorporated by ploughing in the autumn and maize cv. HD 220 sown the f...

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Smith, I.; Mettem, C.J.; Devonport, I., 1984: Towards a rational design of timber structures with mechanical joints. The theory of various methods of estimating joint properties is discussed and an example of sensitivity analysis (believed by the authors to be the most appropriate) is described.