geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Observations on the stability of growth hormones


, : Observations on the stability of growth hormones. Jour Biol Chem 218(1): 115-122

Treatment of growth hormone solutions at or below pH 4.0 and at pH 11.5 or above resulted in the formation of progressively increasing amounts, proportional to the time of exposure, of a fast moving electrophoretic component. At both extremes of pH, biological activity diminished with time of exposure and was lost most rapidly in glycine buffer at pH 2.5. In the range from pH 5.0 to 11.5, no electrophoretic change or loss of activity was observed under the conditions employed. Hence the region of maximal stability of growth hormone was determined to be from pH 5.0 to 11.5. Treatment of growth hormone with 0.1 [image] NaOH gradually converted it to a new electrophoretic component which possessed a higher mobility and thus a higher net charge in both acid and alkaline buffers.

US$29.90

PMID: 13278320


Other references

Nezhat; Nezhat; Borhan, 1996: Laparoscopic Removal of the Cervical Stump after Supracervical Hysterectomy for Persistent Pelvic Pain and Endometriosis. We evaluated the safety of laparoscopic removal of the cervical stump after supracervical hysterectomy. In six women the cervical stump was left due to the presence of severe endometriosis or adhesions in the posterior cul-de-sac between the rectu...

Buchborn, E., 1953: Clinical experiences with parokhellin. Arztliche Wochenschrift 8(16): 388-393

Nardin, M.E.; Pelegri, D.G.; Manias, V.G.; Méndez, E.de.los.A., 2006: Etiological agents of dermatomycoses isolated in a hospital of Santa Fe City, Argentina. Superficial mycoses are limited to skin, hair, nails and mucous membranes. The most common aetiological agents are dermatophytes and yeasts of Candida genus. The aim of this work was to know the aetiological agents of dermatomycoses and their clin...

Anonymous, 2012: Potent Inhibition of Human Sulfotransferase 1A1 by 17alpha-Ethinylestradiol Role of 3-Phosphoadenosine 5-Phosphosulfate Binding and Structural Rearrangements in Regulating Inhibition and Activity. Sulfotransferase (SULT) 1A1 is the major drug/xenobiotic-conjugating SULT isoform in human liver because of its broad substrate reactivity and high expression level. SULT1A1 sulfates estrogens with low micromolar K<SUB>m</SUB> values c...

Verboon-Maciolek, M.A.; Groenendaal, F.; Hahn, C.D.; Hellmann, J.; van Loon, A.M.; Boivin, G.; de Vries, L.S., 2008: Human parechovirus causes encephalitis with white matter injury in neonates. To assess the role of human parechoviruses (HPeVs) as a cause of neonatal cerebral infection and to report neuroimaging findings of newborn infants with encephalitis caused by HPeVs. Clinical presentation, cranial ultrasonography, magnetic resonan...

Wenzel, J.; Shaha, M.; Klimmek, R.; Krumm, S., 2011: Working through grief and loss: oncology nurses' perspectives on professional bereavement. To determine facilitators and barriers to managing patient loss from the combined perspectives of oncology nurses and to extract essential components of a supportive intervention. Qualitative, descriptive. The comprehensive National Cancer Institu...

Kopanska, K.S.; Alcheikh, Y.; Staneva, R.; Vignjevic, D.; Betz, T., 2016: Tensile Forces Originating from Cancer Spheroids Facilitate Tumor Invasion. The mechanical properties of tumors and the tumor environment provide important information for the progression and characterization of cancer. Tumors are surrounded by an extracellular matrix (ECM) dominated by collagen I. The geometrical and mec...

Verbitskiy M.Ya, 1982: The influence of Antarctic and Greenland ice sheets on the level of the world ocean; a numerical experiment. Pages 120-124 1982

Blackman, N.S., 1975: Medical practice and contingent legal fee. New York State Journal of Medicine 75(8): 1295-1298

Sugimoto, S., 1999: Overwintering of rose-infesting aphids (Homoptera: Aphididae) at Wenatchee, Washington State, USA, with redescription of Wahlgreniella nervata (Gillette). The overwintering of aphids infesting roses was investigated in the field at Wenatchee, Washington State (USA), from November 1995 to February 1996. Apterous and/or alate viviparous females, in addition to sexual morphs, of Macrosiphum euphorbiae,...