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Observations on the stability of growth hormones

, : Observations on the stability of growth hormones. Jour Biol Chem 218(1): 115-122

Treatment of growth hormone solutions at or below pH 4.0 and at pH 11.5 or above resulted in the formation of progressively increasing amounts, proportional to the time of exposure, of a fast moving electrophoretic component. At both extremes of pH, biological activity diminished with time of exposure and was lost most rapidly in glycine buffer at pH 2.5. In the range from pH 5.0 to 11.5, no electrophoretic change or loss of activity was observed under the conditions employed. Hence the region of maximal stability of growth hormone was determined to be from pH 5.0 to 11.5. Treatment of growth hormone with 0.1 [image] NaOH gradually converted it to a new electrophoretic component which possessed a higher mobility and thus a higher net charge in both acid and alkaline buffers.


PMID: 13278320

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