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On Physico-Chemical Interactions Between Daunomycin And Nucleic Acids

, : On Physico-Chemical Interactions Between Daunomycin And Nucleic Acids. Biochimica et Biophysica Acta 103: 25-49

1. Daunomycin is a new antibiotic with cytotoxic and antimitotic activity. 2. When cells are incubated in the presence of daunomycin the antibiotic appears to be bound to deoxynucleoproteins, or more precisely, to deoxyribonucleic acid. 3. If native or heat denatured deoxyribonucleic acid from various sources is added to a solution of daunomycin, the antibiotic shows some physico-chemical changes, such as: (a) changes in the visible absorption spectrum, (b) changes in the ultraviolet absorption spectrum, (c) changes in the fluorescent emission, (d) changes in the polarographic behaviour. 4. If deoxyribonucleic acid digested by deoxyribonuclease, apurinic deoxyribonucleic acid, or pyrimidine derivatives, (nucleotides, nucleosides or free bases) are added to a daunomycin solu[long dash]tion, no physico-chemical changes take place; on the contrary some changes in the visible spectrum are caused by purine derivatives (nucleo-tides, nucleosides and free bases) only at very high doses. However, these changes appear to be different from those produced by DNA. 5. Ribonucleic acid also appears to bind with daunomycin, but the physico-chemical changes indicated are not exactly the same as those induced by DNA. 6. If daunomycin is added to a solution of DNA, the nucleic acid shows certain physico-chemical changes such as: (a) changes in the sedimentation coefficient, (b) changes in the viscosity, (c) changes in thermal denaturation-renaturation behaviour, (d) changes in the optical activity. 7. The nature of the bonds between daunomycin and nucleic acids is discussed and consideration is given to the biological consequences of this bond formation.


PMID: 14336441

DOI: 10.1016/0005-2787(65)90539-3

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