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Plant and fungal Fpg homologs are formamidopyrimidine DNA glycosylases but not 8-oxoguanine DNA glycosylases

, : Plant and fungal Fpg homologs are formamidopyrimidine DNA glycosylases but not 8-oxoguanine DNA glycosylases. Dna Repair 8(5): 643-653

Formamidopyrimidine DNA glycosylase (Fpg) and endonuclease VIII (Nei) share an overall common three-dimensional structure and primary amino acid sequence in conserved structural motifs but have different substrate specificities, with bacterial Fpg proteins recognizing formamidopyrimidines, 8-oxoguanine (8-oxoG) and its oxidation products guanidinohydantoin (Gh), and spiroiminodihydantoin (Sp) and bacterial Nei proteins recognizing primarily damaged pyrimidines. In addition to bacteria, Fpg has also been found in plants, while Nei is sparsely distributed among the prokaryotes and eukaryotes. Phylogenetic analysis of Fpg and Nei DNA glycosylases demonstrated, with 95% bootstrap support, a clade containing exclusively sequences from plants and fungi. Members of this clade exhibit sequence features closer to bacterial Fpg proteins than to any protein designated as Nei based on biochemical studies. The Candida albicans (Cal) Fpg DNA glycosylase and a previously studied Arabidopsis thaliana (Ath) Fpg DNA glycosylase were expressed, purified and characterized. In oligodeoxynucleotides, the preferred glycosylase substrates for both enzymes were Gh and Sp, the oxidation products of 8-oxoG, with the best substrate being a site of base loss. GC/MS analysis of bases released from -irradiated DNA show FapyAde and FapyGua to be excellent substrates as well. Studies carried out with oligodeoxynucleotide substrates demonstrate that both enzymes discriminated against A opposite the base lesion, characteristic of Fpg glycosylases. Single turnover kinetics with oligodeoxynucleotides showed that the plant and fungal glycosylases were most active on Gh and Sp, less active on oxidized pyrimidines and exhibited very little or no activity on 8-oxoG. Surprisingly, the activity of AthFpg1 on an AP site opposite a G was extremely robust with a kobs of over 2500 min−1.


PMID: 19217358

DOI: 10.1016/j.dnarep.2008.12.013

Other references

Jorgensen, M., 1971: A case of abnormal intrahepatic bile duct arrangement submitted to three dimensional reconstruction. Acta Pathologica et Microbiologica Scandinavica. Section A, Pathology 79(3): 303-306

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Ballarino, M.; Rumolo, R., 1968: The mechanism of initiation of supraventricular paroxysmal tachycardia. Atti Della Societa Italiana di Cardiologia 2: 134-135

Deschenes, M.; Bourassa, J.; Parent, A., 1995: Striatal and cortical projections of single cells from the central lateral thalamic nucleus in the rat. Society for Neuroscience Abstracts 21(1-3): 1188

Bennett, J.W., 2016: The fungi that ate my house. Science 349(6251): 1018-1018

Sonnenberg, G.F.; Fouser, L.A.; Artis, D., 2011: Functional biology of the IL-22-IL-22R pathway in regulating immunity and inflammation at barrier surfaces. Expression of interleukin (IL)-22, a member of the IL-10 cytokine family, has recently been reported in a number of human diseases, including mucosal-associated infections and inflammatory disorders of the intestine, skin, and joints. Both T cells...

Larsen, R.I.; Mccurdy, T.R.; Johnson, P.M.; Heck, W.W., 1988: An air quality data analysis system for interrelating effects standards and needed source reductions part 10. potential ambient ozone standards to limit soybean crop reduction. Soybean percent crop reduction was estimated as a function of ambient O3 concentrations for each of 80 agricultural sites in the National Aerometric Data Bank (NADB) for each available year of data for years 1981-1985. Fourteen O3 concentration st...

Battke, F.; Symons, S.; Nieselt, K., 2010: Mayday--integrative analytics for expression data. DNA Microarrays have become the standard method for large scale analyses of gene expression and epigenomics. The increasing complexity and inherent noisiness of the generated data makes visual data exploration ever more important. Fast deployment...

Petrov, A.; Burke, N.; Neely, C.M.; Robinson, M.L.; Watkins, S.; Ameredes, B.T.; Calhoun, W.J., 2003: Quantification of glucocorticoid receptor activation and translocation A novel method. Journal of Allergy & Clinical Immunology 111(2 Abstract Supplement): S113, February