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Purification and chemistry of bacteriophage

, : Purification and chemistry of bacteriophage. J Virol 1(3): 561-598

From a stock of [CHI] phage grown on Salmonella, a host-range mutant which attacks Escherichia coli was isolated. As in the case of Salmonella, only motile strains of E. coli are sensitive to [CHI]. The phage shows an eclipse period of 35 min and a minimal latent period of 52 min. The adsorption rate constant is 3 x 10-9 mi/min. Adsorption shows a marked dependence on temperature. Bacteriophage [CHI] was purified by differential centrlfugation and CsCl density gradient centrifugation. It contains deoxyribonucleic acid (DNA) which is double-stranded. The DNA has a molecular weight of 42 million and a guanine plus cytosine content of 57%. Of 68 molecules of DNA inspected, 7 were circular. The phage particle weight is about 90 million.


PMID: 4918240

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