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Selective demonstration of histidine


, : Selective demonstration of histidine. Stain Technology 31(5): 197-200

Mounted paraffin sections of formalin-fixed tissue are treated for 24 hr at room temperature in an iodine solution (0.3% iodine, 0.6% potassium iodide) at pH 10 to block the aromatic nuclei of tyrosine and tryptophane. A coupled tetrazonium reaction using naphthanil diazo blue B (tetrazotized o-dianisidine) as a 0.1% solution at pH 9.2 for 15 min at 4[degree] C, as the first coupling agent, and H acid (8-amino-l-naphthol-3, 6-dissulfonic acid), as a 2% solution at pH 9.2 for 15 min at 4[degree] C, as the second coupling agent, stains sites of histidine a red-brown to red-purple color.

US$19.90

PMID: 13360484

DOI: 10.3109/10520295609113804


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