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Separation of plasma thromboplastin antecedent and Hageman factor from human plasma


, : Separation of plasma thromboplastin antecedent and Hageman factor from human plasma. Proc Soc Exptl Biol And Med 105(3): 453-455

Plasma thromboplastin antecedent (PTA) and Hageman factor (HF) have been separated from human plasma by ion exchange chromatography using diethylaminoethyl cellulose resin. A constant pH of 7.12 was used; salt concentration was increased continuously from 0.05 [image] to 0.3 [image] by varying NaCl concentration in presence of 0.02 [image] sodium diethyl barbiturate buffer. The maximum PTA activity concided with the first protein peak while the greatest HF activity immediately preceded the second protein maximum. Each fraction contained less than 1% of normal of all other known clotting factor activities. Each purified fraction corrected the defect of its corresponding deficiency plasma. However, the combined purified factors failed to shorten the clotting time of "exhausted plasma" (prepared by Waaler's technique of treating normal plasma with celite powder) unless another as yet unidentified component of plasma was also present.

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Other references

Logan, C.; Coaker, T.H., 1960: The transmission of bacterial blight of Cotton (Xanthomonas malvacearum (E. F. Smith) Dowson) by the Cotton bug, Lygus vosseleri Popp. In experiments at Namulonge, Uganda, adult L. vosseleri disseminated X, malvacearum to healthy cotton plants in polythene bags in the glasshouse. Typical damage resulted on all such plants. When 2 plants that had been caged with non-contaminated i...

Rees, P.H., 1969: Electrocardiographic patterns in endomyocardial fibrosis of the right ventricle. Transactions of the Royal Society of Tropical Medicine and Hygiene 63(1): 24-24

Loffer, F.D., 1966: Thalassemia minor associated with pregnancy. Report of a case and brief discussion of hemoglobinopathies. Obstetrics and Gynecology 27(4): 532-535

Nichols, L.A., 1960: Respiration and the emotions. Medical World 92: 129-133

Greshake, B., 2017: Looking into Pandora's Box: The Content of iSci-Hub and its Usage. Despite the growth of Open Access, potentially illegally circumventing paywalls to access scholarly publications is becoming a more mainstream phenomenon. The web service Sci-Hub is amongst the biggest facilitators of this, offering free access to...

Ishida, A.; Toma, T.; M.M., 2003: Leaf gas exchange and cholorphyll fluorescence in relation to leaf angle, azimuth, and canopy position in the tropical pioneer tree, Macaranga conifera. We tested the hypothesis that, in tropical pioneer tree species, vertical leaf angle contributes to high carbon gain because it minimizes damage caused by high irradiances. Diurnal changes in leaf gas exchange and chlorophyll fluorescence were mea...

Neubert, R.; Fritsch, B.; Dongowski, G., 1995: Interactions between food components and drugs. 3. Interactions between pectin and propanolol. In an in vitro study, the influence of the soluble fibre pectin on transport of propanolol through an artificial lipid membrane was examined, using a 2-compartment model with a dodecanol-collodium membrane at pH 7.2 and 37 degrees C. 2 series of d...

Park, S-Woong.; Choi, J-Min.; Lee, K.Hyun.; Yeom, H.Woong.; Im, S.; Lee, Y.Kook., 2010: Amorphous-to-crystalline phase transformation of thin film rubrene. We report on the amorphous-to-crystalline phase transformation of rubrene thin films. The crystallization of the organic thin films displays disk-like domains whose nucleation and growth follow phase transformation kinetics well-established for in...

García-Arumí, J.; Ramsay, L.S.; Guraya, B.C., 2000: Transpupillary thermotherapy for circumscribed choroidal hemangiomas. Various treatments have been described for circumscribed choroidal hemangiomas. We present our results with transpupillary thermotherapy (TTT) with infrared laser that indicate that the therapy is a new, effective treatment for this pathologic con...

Shekhtman, E.M., 1958: Anemia in pregnancy due to infestation by Diphyllobothrium latum. Akusherstvo i Ginekologiia 34(4): 39-41