+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Structure And Properties Of Active Ribosomes Of Escherichia Coli

, : Structure And Properties Of Active Ribosomes Of Escherichia Coli. Journal of Molecular Biology 7: 350-359

After protein synthesis in extracts of Escherichia coli stops, about two-thirds of the nascent protein remains attached to the ribosomal surface on which it has been synthesized. The unreleased protein, most likely incomplete chains, stabilizes the binding of 30 s to 50 s ribosomes. Thus, when the Mg2+ concentration is lowered, those 70 s ribosomes bearing nascent protein dissociate more slowly into their 30 s and 50 s subunits. However, dialysis against very low Mg2+ (5 x 10-5 M) releases the nascent protein from the ribosomes. When the Mg2+ concentration is again raised, the nascent protein chains return to the ribosomes, probably linked to molecules of soluble ribonucleic acid (RNA). Since this treatment leaves intact the 50 s and 30 s subunits, covalent bonds are probably not involved in stabilizing the attachment of nascent protein to the ribosomes. Rather, the component messenger RNA, 30 s and 50 s ribosomes and nascent protein of a functioning ribosome appear to be held together by the combined strength of a number of weak bonds, including cationic bridges between molecules of RNA. Pulse-labeled messenger RNA is similarly released from ribosomes by dialysis against very low Mg2+; this messenger RNA is again bound when the Mg2+ concentration is raised. The functional ribosomes, thus renewed, have not been damaged: they form protein to the same extent as in control extracts kept in high Mg2+.


PMID: 14066613

DOI: 10.1016/S0022-2836(63)80029-7

Other references

Thompson, P.E., 1972: Joint 1972 tropical medicine and parasitology meetings in Miami. Archivos de Investigacion Medica 2: Suppl 2:239-40

Parak, R.B.; Wadee, A.A., 1991: The synergistic effects of gamma interferon and clofazimine on phagocyte function: restoration of inhibition due to a 25 kilodalton fraction from Mycobacterium tuberculosis. A 25 kDa glycolipoprotein from Mycobacterium tuberculosis has been shown to inhibit phagocyte functions associated with antimicrobial activity. These include the intracellular killing ability, reduction of nitro blue tetrazolium, hydrogen peroxide...

Kabanov, A.V.; Chekhonin, V.P.; Alakhov VYu; Batrakova, E.V.; Lebedev, A.S.; Melik-Nubarov, N.S.; Arzhakov, S.A.; Levashov, A.V.; Morozov, G.V.; Severin, E.S., 1989: The neuroleptic activity of haloperidol increases after its solubilization in surfactant micelles. Micelles as microcontainers for drug targeting. It has been suggested to use surfactant micelles as microcontainers for increasing the efficiency of neuroleptic targeting from blood flow into the brain. The neuroleptic action of haloperidol, intraperitoneally injected into mice in micellar solu...

Griffin, D.; Brown, L.; Feinn, R.; Jacob, M.Casey.; Scranton, V.; Egan, J.; Nulsen, J., 2012: Impact of an educational intervention and insurance coverage on patients' preferences to transfer multiple embryos. Multiple gestations resulting from IVF continue to be a major problem associated with maternal/neonatal morbidity and mortality including preterm labour/delivery, pre-eclampsia and post-partum haemorrhage. A prospective survey at a university IVF...

Bozzini S.; Visai L.; Pignatti P.; Petersen T.E.; Speziale P., 1992: Multiple binding sites in fibronectin and the staphylococcal fibronectin receptor. The binding of fibronectin to Staphylococci exhibits the properties of a ligand-receptor interaction and has been proposed to mediate bacterial adherence to host tissues. To localize staphylococcal-binding sites in fibronectin, the protein was sub...

Rachev, A.I., 1980: Effects of transmural pressure and muscular activity on pulse waves in arteries. Propagation of small amplitude harmonic waves through a viscous incompressible fluid contained in an initially stressed elastic cylindrical tube is considered as a model of the pulse wave propagation in arteries. The nonlinearity and orthotropy of...

Siostrzonek P.; Koppensteiner R.; Gottsauner Wolf M.; Heinz G.; Kreiner G.; Pacher R.; Frey B.; Goessinger H., 1992: Hemodynamic and rheologic determinants of left atrial spontaneous echo contrast in idiopathic dilated cardiomyopathy. European Heart Journal 13(ABSTR SUPPL): 283

D.M.rcoyrol, L.; Job, C.; Job, D., 1989: Effect of Sarkosyl and heparin on single-step addition reactions catalysed by wheat-germ RNA polymerase II--poly[d(A-T)]transcription complexes. Incubation of purified wheat-germ RNA polymerase II with poly[d(A-T)] template, Mn2+, U-A dinucleoside monophosphate primer and UTP substrate resulted in catalytic formation of the trinucleoside diphosphate U-A-U, in accordance with the results of...

Flens, G.; Smits, N.; Terwee, C.B.; Dekker, J.; Huijbrechts, I.; de Beurs, E., 2017: Development of a Computer Adaptive Test for Depression Based on the Dutch-Flemish Version of the PROMIS Item Bank. We developed a Dutch-Flemish version of the patient-reported outcomes measurement information system (PROMIS) adult V1.0 item bank for depression as input for computerized adaptive testing (CAT). As item bank, we used the Dutch-Flemish translation...

Genel M.; Pearson H.; O'brien R.; Hintz R., 1976: Patho physiology of growth failure in thalassemia major. Pediatric Research 10(4): 376