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Studies on the initial phase of poliovirus reproduction cycle


, : Studies on the initial phase of poliovirus reproduction cycle. Acta Microbiol Acad Sci Hung 13(1): 85-94

Type 1 poliovirus (strain Mahoney) and a permanent monkey kidney cell line (PMK III/1) were used throughout. In Parker's No. 199 medium this system produced full virus yields whether or not bovine albumin had been added. To obtain full virus yield in Hanks' balanced salt solution the presence of bovine albumin was indispensable and its omission resulted in a 90% or even greater reduction of the amount of virus produced. Presence of bovine albumin in Hanks' balanced salt solution was required only during the first 30 min. of the cycle, while its addition after 30 to 60 min. caused 50 and 80% reduction of the yield, respectively. Bovine albumin added in the 2nd hr. of the cycle or later did not increase the yield above that obtained in Hanks' balanced salt solution alone. Absence or delayed addition (2 hr. or later) of bovine albumin caused a 30 to 40 min. retardation of the cycle's onset in Hanks' balanced salt solution. Replication rate was practically identical in all cases, duplication time being approximately 10 min. Bovine albumin seems to be a factor involved in the initial mechanisms of viral infection (penetration or eclipse). Its probable role in the "virtual eclipse" of adsorbed virions is discussed. A closer approach to the possible mechanism of bovine albumin action was attempted by studying the effect of cysteine and glutathione and of K linoleate on the initial phase of viral cycle.

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PMID: 4288239


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