Technique for the preservation of tetracycline fluorescence in ascites tumor cells
Barbour, E.P.; Thompson, J.R.; Riedesel, C.C., 1967: Technique for the preservation of tetracycline fluorescence in ascites tumor cells. Journal of Pharmaceutical Sciences 56(2): 272-274
Fluorescence microscopy has a potential application in cellular pharmacology for the study of intra- and intercellular distribution of pharmacological agents. A method is reported for preparing permanent preparations of tetracycline-retaining ascite tumor cells. A combination of glutaral-dehyde fixation followed by glycerol dehydration was found satisfactory.