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Techniques of accelerated detection of typhoid and dysentery bacteria in water Problems of sanitary bacteriology and virology


, : Techniques of accelerated detection of typhoid and dysentery bacteria in water Problems of sanitary bacteriology and virology. Techniques of accelerated detection of typhoid and dysentery bacteria in water Problems of sanitary bacteriology and virology K metodike uskorennoi indikatsii bakterii bryushnogo tifa i dizenterii v vode In: Voprosy sanitarnoi bakteriologii i vlrusologii Problems of sanitary bacteriology and virology 166-170

The problems involved in the study of water for the presence of pathogenic bacteria of the coli group is discussed. The concentration of the bacteria may be shortened to a few min. by using membrane or absorption filters of the FPP-5 type. Increasing the bio mass of the bacteria is achieved by culturing on semiliquid nutrient media or in liquid media with the application of aeration and biostimulators (2 to 3 ml of oats sprout extract/10-12 ml of medium). Intensive growth of the pathogenic bacteria can be observed in 8-10 hrs. at 39-40[degree] C when this is done. The biochemical activity of the bacteria can be checked by sowing them in differential media with increased indicator content and pre-heated to 39[degree] C. A clear response is obtained in 3-5 hrs under these conditions. Serological determination o the strains is done by slide reaction. The time necessary to get a final response can be shortened from 3-4 days to 18 hrs.

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