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The determination of tryptophane as a measure of the globulin content of serum


, : The determination of tryptophane as a measure of the globulin content of serum. Zeitschr Klin Med 110(2): 224-240

The author reviews the methods for the determination of the serum proteins and concludes that precipitation by neutral salts is most satisfactory, if done under proper conditions. A rapid method is described that depends upon the determination of tryptophane by the colorimetric method of Fiirth and Dische, and the known contents of tryptophane in serum globulin, 3.9%, and in serum albumin, 1.4%. The normal content of tryptophane is 2-3%, values above 3% indicating pathological increase in serum globulin. The method was applied to nearly 300 cases. All cases with increased tryptophane content showed also increased sedimentation time and increased serum viscosity, but over 30% of the cases showing increased sedimentation time showed normal content of tryptophane. In a few cases the tryptophane content was greater than 4%, indicating a different chemical composition of the plasma proteins.

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