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The distribution of nitrogen in the molecule of vitamin B12


, : The distribution of nitrogen in the molecule of vitamin B12. Iowa State Coll Jour Sci 30(2): 269-276

Application of the Plimmer modification of the Van Slyke amino nitrogen determination has shown the presence of 5 amide groups in vitamin B12. A quantitative determination of the hydrolysis products of vitamin B12 has shown that 5 molecules of NH3 are liberated, that 2 N atoms are present in a form which does not yield NH3 on acid hydrolysis but does yield NH3 on treatment with periodic acid (one or both possibly 1,2-aminoalcohol), and that the N to Co ratio in the red acidic fragment is 4.

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