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The effect of adrenal cortical hormones on the incorporation of C14 into protein of lymphatic cells


, : The effect of adrenal cortical hormones on the incorporation of C14 into protein of lymphatic cells. Endocrinology 52(1): 1-9

The respiration of lymphatic cells obtained from rabbit appendix, rat and mouse spleen, rat thymus, and from the Gardner mouse lymphosarcoma was slightly inhibited by 17-hydroxycorticosterone and by 17-hydroxy-11-dehydrocorticosterone. Oxidation of some of the intermediates of the tricarboxylic acid cycle was also little affected, whereas there was definite inhibition of the anaerobic glycolysis of appendix lymphatic cells. The C14 incorporation into the protein of the normal lymphatic cells on addition of C14 labeled glycine, acetate, and DL-phenylalanine was inhibited by the adrenal hormone. Incorporation into the proteins of lymphosarcoma cells was not affected by hormone addition similar to that used in normal cells, a higher concn. of hormone being required to obtain inhibition.

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