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The effect of magnesium chloride and pH on heat in-activatipn of the fattv acid oxidase of mitochondria


, : The effect of magnesium chloride and pH on heat in-activatipn of the fattv acid oxidase of mitochondria. Proc Soc Exptl Biol And Med 87(2): 465-469

A study was made of the factors in the complete reaction mixture which protect the fatty acid oxidase of rat liver mitochondria from inactivation by heating at 30[degree]. It was found that the alkaline pH of the reaction mixture and the presence of MgCl were the most important factors in the prevention of the heat inactivation of the sorbate, alpha-ketoglutarate, or malate oxidase of rat liver mitochondria. The most destructive factor was the presence of phosphate. Either MgCl or adenosine triphosphate (ATP) prevented, and phosphate accelerated, the destruction of mitochondria in hypotonic solution, but MgCl had a specific protective effect on sorbic acid oxidase in addition to its effect of preventing destruction of the mitochondria.

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