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The incorporation in vitro of radioactive phosphorus into the proteins of rat liver supernatant


, : The incorporation in vitro of radioactive phosphorus into the proteins of rat liver supernatant. Acta Societatis Medicorum Upsaliensis 64: 236-243

Rat liver supernatant proteins were fractionated by precipitation with ammonium sulfate at 50 and 85% saturation (fractions AS-50 and AS-85). After incubation with P32-labeled adenosinetriphosphate (ATP32), active proteins were mainly present in AS-50. The incorporation of P32 in AS-50 proteins is dependent upon the presence of Mg; 0.05 [image] ethylenediaminetetraacetic acid completely inhibited the reaction. This type of incorporation may be attributable to the action of a phospho-protein phosphokinase or a phosphotransferase involving ATP. Labeled phosphorylserine was demonstrated in acid hydrolysates of 2 protein subfractions of AS-50. When the 2 main fractions were incubated with p32o4 rather than with ATP32, AS-85 incorporated most of the activity. This incorporation was not Mg-dependent, showed a pH optimum between 7 and 8, and was not Mg-dependent, showed a pH optimum between 7 and 8, and was abolished by heating 2 min. at 100[degree]. An isolated unidentified protein from AS-85 was also found to incorporate P32; hydrolysates of this protein contained serine-P32. This protein was not alkaline phosphatase, since 99-100% of this activity was present in AS-50.

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PMID: 13828488


Other references

Antipin V.S., 1977: Scheme of Mesozoic magmatism in the Dzhidinsk region, Southwest Transbaikalia. Pages 60-65 1977

Nover, A., 1961: On the effect of light and diathermy coagulations on the retina, choroid and sclera of the rabbit. Klinische Monatsblatter für Augenheilkunde und für Augenarztliche Fortbildung 138: 41-53

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Tottman, D.R.; Thompson, W., 1978: The influence of herbicides on the incidence of take-all disease (Gaeumannomyces graminis) on the roots of winter wheat. Herbicides widely used to control broad-leaved weeds in cereal crops increased natural low levels of take-all infection on the roots of winter wheat. In field experiments, mecoprop, ioxynil, dicamba and a dicamba, 2,3,6-TBA, mecoprop and MCPA mixt...

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Ricklefs R.E., 1975: Patterns of growth in birds part 3 growth and development of the cactus wren. Condor 77(1): 34-45

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