+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The incorporation in vitro of radioactive phosphorus into the proteins of rat liver supernatant

, : The incorporation in vitro of radioactive phosphorus into the proteins of rat liver supernatant. Acta Societatis Medicorum Upsaliensis 64: 236-243

Rat liver supernatant proteins were fractionated by precipitation with ammonium sulfate at 50 and 85% saturation (fractions AS-50 and AS-85). After incubation with P32-labeled adenosinetriphosphate (ATP32), active proteins were mainly present in AS-50. The incorporation of P32 in AS-50 proteins is dependent upon the presence of Mg; 0.05 [image] ethylenediaminetetraacetic acid completely inhibited the reaction. This type of incorporation may be attributable to the action of a phospho-protein phosphokinase or a phosphotransferase involving ATP. Labeled phosphorylserine was demonstrated in acid hydrolysates of 2 protein subfractions of AS-50. When the 2 main fractions were incubated with p32o4 rather than with ATP32, AS-85 incorporated most of the activity. This incorporation was not Mg-dependent, showed a pH optimum between 7 and 8, and was not Mg-dependent, showed a pH optimum between 7 and 8, and was abolished by heating 2 min. at 100[degree]. An isolated unidentified protein from AS-85 was also found to incorporate P32; hydrolysates of this protein contained serine-P32. This protein was not alkaline phosphatase, since 99-100% of this activity was present in AS-50.


PMID: 13828488

Other references

Antipin V.S., 1977: Scheme of Mesozoic magmatism in the Dzhidinsk region, Southwest Transbaikalia. Pages 60-65 1977

Nover, A., 1961: On the effect of light and diathermy coagulations on the retina, choroid and sclera of the rabbit. Klinische Monatsblatter für Augenheilkunde und für Augenarztliche Fortbildung 138: 41-53

Yaris, N.; Kutluk, T., 1998: Free radicals and cancer. Free radicals are chemical species possessing and unpaired electron and are generally very reactive in the biological system. Their major source in cells in electron leakage from the electron transport chain. Radicals are deliberately generated by...

Saokaew, S.; Khaisombat, N.; Chaiyakunapruk, N.; Likittanasombat, K.; Nathisuwan, S., 2012: Attributable Cost and Length of Stay for Patients with Enoxaparin-Associated Bleeding. Patients receiving enoxaparin are at risk of bleeding. The study of the economic impact of enoxaparin-associated bleeding in Asian population, however, is limited. This study aimed to estimate the attributable costs and length of stay (LOS) of pat...

Tottman, D.R.; Thompson, W., 1978: The influence of herbicides on the incidence of take-all disease (Gaeumannomyces graminis) on the roots of winter wheat. Herbicides widely used to control broad-leaved weeds in cereal crops increased natural low levels of take-all infection on the roots of winter wheat. In field experiments, mecoprop, ioxynil, dicamba and a dicamba, 2,3,6-TBA, mecoprop and MCPA mixt...

Wedzony, K.; Czyrak, A., 1994: The role of corticosteroids in the acquisition of sensitization to locomotor stimulant effects of MK-801. In the present study, we investigated the role of corticosterone and glucocorticoid receptors in the acquisition of sensitization to locomotor stimulant effects of MK-801 in rats. MK-801 (two doses, 0.4 mg/kg i.p. each, given twice, 48 h apart) ev...

Ricklefs R.E., 1975: Patterns of growth in birds part 3 growth and development of the cactus wren. Condor 77(1): 34-45

Liu, W.T.o; Mino, T.; Nakamura, K.; Matsuo, T., 1996: Glycogen accumulating population and its anaerobic substrate uptake in anaerobic-aerobic activated sludge without biological phosphorus removal. The presence of a glycogen accumulating population and its abilities of substrate uptake and storage in anaerobic-aerobic activated sludge fed with mainly acetate were investigated. Because a low phosphorus/carbon feeding ratio (2/100, wt/wt) was...

Beltran, J.; Jbara, M.; Maimon, R., 2003: Shoulder: labrum and bicipital tendon. Shoulder instability can be due to a single, acute traumatic event, generalized joint laxity, or repeated episodes of microtrauma. The later occurs in the throwing athlete. The most common lesion involving the labrum is the anterior inferior labra...

Meader, N.; Chan, M.K.Y., 2017: Sexual orientation and suicidal behaviour in young people. Lesbian, gay, and bisexual (LGB) young people have been found to be at greater risk of suicidal behaviour. National prevention strategies have identified the need to reduce suicide risk in this population. However, research on specific risk factor...