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The isolation and characterization of an enterovirus of plgs

, : The isolation and characterization of an enterovirus of plgs. Wiener Tierarztl Monatsschr 52(7): 675-685

A cytopathic agent was isolated in pig testis cell cultures inoculated with material from the spleen of a pig with notorious swine fever infection. Owing to the origin of this agent (spleen of a pig infected with swine fever) a thorough characterization was made. The virus resisted chloroform, ether, saponin and trypsin; no inhibition of growth was obtained by guanidine, HBB and 5-iodo-deoxyuridine. In pathogenicity tests on animals the virus was not pathogenic for laboratory animals, serological-ly negative piglets and S. P. F. pigs. On the ground of physical-chemical and biological characteristics the virus was classified as enterovirus. Pig testis cell cultures were found very suitable for isolation, characterization and serological examination of enteroviruses. 35 ECSO virus strains have been isolated so far in pig testicular cell cultures. The isolated strains showed different serological reactions. Thus 7 strains were classified into two distinct serological groups by cross-neutralization tests.


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