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The quantitative relation of fibrinolysin and antifibrinolysin

, : The quantitative relation of fibrinolysin and antifibrinolysin. Jour Immunol 36(1): 45-58

A dried, 3 volume 95% alcohol precipitate of a 14-hour culture filtrate of a hemolytic Streptococcus was dissolved in buffer solns. in concs. varying at 0.02% increments from 0.2% to 0.02% and used as a source of fibrinolysin. 056 cc. volumes of these solns. were used for titrating 6 concs. of a hemolytic streptococcus pharyngitis convalescent serum in the presence of 02 cc. of a single susceptible human plasma. The time required for complete lysis of the plasma clots was recorded. Since the plasma source and conc. were uniform, there were three major variables in this experiment: namely, the fibrinolysin conc. the serum (antifibrinolysin) conc. and the time required for lysis of the clots. Each of these variables was successively considered as a constant while the remaining 2 were plotted against each other. The resulting curves suggest that there is a quantitative neutralization of a given fibrinolysin preparation by a given serum (antifibrinolysin) under the conditions of the experiment.


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