geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The ultracentrifugal protein sedimentation diagram of normal human, cow and horse serum


, : The ultracentrifugal protein sedimentation diagram of normal human, cow and horse serum. Biochemical Journal 29(3): 660-693

The behavior of untreated human, cow and horse sera in the ultracentrifuge was studied to detn. the molecular dimensional state of the native serum proteins. The serum proteins of these 3 spp. are constituted in a similar manner: 3 molecular types, of which 2 correspond in sedimentation velocities to purified horse serum albumin and globulin; the 3d (the chemical nature of which is not known and which may not be capable of existence apart from the other serum proteins) has a molecular wt. slightly greater than albumin and considerably less than globulin. Photographs taken some time after sedimentation has begun, when the protein boundaries are separated sufficiently from each other, may be translated into refractive index diagrams which picture the protein composition of the serum and, in particular, give the conc. of each fraction and a measure of the degree of molecular homogeneity of the lightest. The sedimentation diagram is a strictly reproducible characteristic of a serum. Dialysis of cow serum against 1% NaCl or acetate buffer, or heating at 56[degree] for some hrs. causes no change. The diagram of redissolved serum powder shows the presence of considerable amts. of polydisperse protein. Treatment with (NH4)SO4 or with alcohol at low temps. gives rise to an irreversible change in the diagram. Simple dilution of cow, horse and human sera results in an increase in the conc. of globulin at the expense of the lighter fractions. The diagram of horse plasma shows that the fibrinogen is not present as a fraction of unusual molecular wt. It is not certain in which of the normal molecular fractions it is included. In sera from 10 normal persons the diagrams are closely similar[long dash]there are only small differences in the concs. of the fractions. The proportions of globulin are 5-15% of the total protein. The relationship between the ultra-centrifugal results for normal sera and the colloid osmotic pressures of undiluted sera is discussed.

US$19.90

PMID: 16745713

DOI: 10.1042/bj0290660


Other references

Pavlović, E.; Peitl, M.Vucić.; Proloscić, J.; Petrić, D., 2015: Virus etiology of schizophrenia: the characteristics of the "*Little Ice Age" and its consequences. The data of the "Little Ice Age" (1500-1850) in Croatia and those which applied to human health were especially emphasized were analyzed. They are some which stand out like: importance of the sort of soil and relief, the influence of cut...

Schloß, S.; Neff, A.Christina.; Becker, K.; Pauli-Pott, U., 2016: Executive Functions in Preschool Children with Increased Risk of ADHD: The Role of Mother-Child Relationship. Praxis der Kinderpsychologie und Kinderpsychiatrie 65(6): 406-422

Daniels, J.T.; Harris, I.R.; Kearney, J.N.; Ingham, E., 1995: Calcium: a crucial consideration in serum-free keratinocyte culture. This investigation was conducted when previously repeatable experimental data became impossible to reproduce when using keratinocytes cultured in serum-free medium. Differences in calcium molarity between batches of medium were identified as a sou...

Hayashi; Furutsuka; Shiono, 1999: Okaramines H and I, new okaramine congeners, from aspergillus aculeatus. Two new congeners of okaramine, okaramines H and I, were isolated from okara (the water-insoluble residue of soyabeans) fermented with A. aculeatus KF-428. Their structures were elucidated by spectroscopic methods. Neither compound showed activity...

Barnhart, K.T.; Stolpen, A.; Pretorius, E.S.; Malamud, D., 2001: Distribution of a spermicide containing Nonoxynol-9 in the vaginal canal and the upper female reproductive tract. Topical, intravaginal microbicides and spermicides are greatly needed to prevent transmission of sexually transmitted diseases and/or unwanted pregnancies. The development of such compounds is a high research priority. The presumed method of actio...

Goldsmith R.S.; Fournier A.; Johnson W.J.; Arnaud C.D., 1970: Etiologic role of dialysis in stimulating hyper parathyroidism during chronic hemo dialysis. Journal of Clinical Investigation 49(6): 35A-36A

Schouppe, A. von., 1954: Xeue Fossilfunde in dcr Lurgrotte bei Peggau. Mitteilungen der Naturwissenschaftlichen Vereins fuer Steiermark. 1951; 79-80

Anonymous, 1992: Grassroots environmental action: people's participation in sustainable development. This book deals with the threat of environmental degradation in the Third World, and with the wide range of actions people take at the local level to manage and protect their natural resources, activities which have the potential to help reverse,...

Dermont, T.; Morizot, L.; Bouhaddi, M.; Ménétrier, A., 2015: Changes in Tissue Oxygen Saturation in Response to Different Calf Compression Sleeves. Aim. The purpose was to examine the changes in tissue oxygen saturation (StO2) in response to the application of different commercially available calf compression sleeves. Methods. Eight subjects came to the laboratory to complete a session in sea...

Merghany, M.M., 1998: Effect of irrigation systems and regimes in relation to farmyard manure levels on potato yield and quality in new reclaimed sandy soils. A field experiment was conducted in Salhyia, Egypt, during the autumn of 1993/94 and 1994/95 to study the effects of irrigation system (sprinkler, drip or subsurface drip irrigation), water regime (2 or 4 litres/m2) and farmyard manure (30 or 60 m...