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Uber die Farbbarkeit der fixierten Zellstrukturen


, : Uber die Farbbarkeit der fixierten Zellstrukturen. Sci Repts Tokyo Bunrika Daigaku Sect B 1(1): 1-21

Nuclear stains are considerably influenced by various fixatives. In general, HgCl2, alcohol and formol enhance staining; trichlora-cetic acid and HNO3 always lessen the stain reaction. Fixation with acetic acid and alcohol most often results in a pure nuclear stain (without staining the cytoplasm at the same time). In staining the nucleus the property of the killing agent to fix primarily the nucleic acid is mainly responsible for the staining. In cytoplasmic staining the degree of dispersion of the fixed plasma structures plays a part. The pH of the fixative seems to influence considerably the staining of cell structures. Thymo-nucleic acid represents the main constituent of the nucleus in root tip cells. The nucleolar substance contains no nucleic acid. The phase boundaries of the protoplasm preserve in their fixed state their lipoid nature. Fixatives containing HgCl2 and bichromate that fix the chondriosomes confer a resistance to Javelles's fluid on the cell structures which depends upon the property of the fixatives to fix the lipoid component as well as the protein part of the protoplasmic structure.

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