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Ultrastructure of basidio-spores and mycelium of Lenzltes saepiarial


, : Ultrastructure of basidio-spores and mycelium of Lenzltes saepiarial. J Bacteriol 92(4): 1218-1227

Ungerminated and germinated basidiospores and 2-day-old mycelial cultures of Lenzites saepiaria were similar in their fine structure. Fixation with glutaraldehyde, followed by osmium tetroxide, was far superior to permanganate. Cell organelles were seen in cytoplasm of spores and hyphae, and clamp connections were abundant in hyphal elements. Numerous lomasomes, vesicular bodies, and to complex concentric membranes occurred in the cytoplasm and were often associated with the cell membrane or the dolipore membrane (parenthesome) of the septum. Endoplasmic reticulum was not found, but numerous ribosomes were seen; polyribosome groupings were frequently noted. The nucleus was bounded by a double membrane which contained few pores. Germinating spores exhibited one or more large OBmiophilic bodies in association with a vacuole and membranous elements. Other than possessing a thin wall, the emerging germ was similar in structure to the parent spore.

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