geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Utilization of amino acids, peptides, and protein by cultures of embryonic heart


, : Utilization of amino acids, peptides, and protein by cultures of embryonic heart. Journal of the National Cancer Institute 14(3): 519-525

Twenty-five percent chick-embryo extract, used as the nutrient medium for the cultivation of chick heart fibroblasts, contained approx. 100 mg. of nonprotein amino acids/100 ml. Almost 25% of these were in conjugated form. During cultivation both free and conjugated amino acids, as well as protein, were removed from the medium. When labeled embryo extract containing C14-amino acids, peptides, and protein was employed, it was possible to equate C14 losses from these fractions with corresponding gains by the heart cultures. Partial proteolytic digests and complete acid hydro-lysates of Cl4-labeled protein were rapidly incorporated into tissue cultures, and at approx. equal rates, when added to the nutrient medium.

US$29.90

PMID: 13233810


Other references

Reller, M.D.; Donovan, E.F.; Kotagal, U.R., 1985: Influence of airway pressure waveform on cardiac output during positive pressure ventilation of healthy newborn dogs. During positive pressure ventilation, increases in mean airway pressure produced by increases in end expiratory pressure are associated with reductions in cardiac output. Mean airway pressure may be increased not only by increasing end expiratory...

Jakus, R., 1995: Bark beetles (Scolytidae) and factors damaging ecological stability of mountainous spruce forests in the regions affected by air pollution. Immissions, some interventions in forest stands and other human activities in mountainous spruce (Picea) forests cause the accumulation of material attractive for bark beetles, decrease the resistance of living trees, xerothermization of environme...

Haralampus-Grynaviski, N.M.; Lamb, L.E.; Clancy, C.M.R.; Skumatz, C.; Burke, J.M.; Sarna, T.; Simon, J.D., 2003: Spectroscopic and morphological studies of human retinal lipofuscin granules. The emission properties of ocular lipofuscin granules isolated from human retinal pigment epithelial cells are examined by using steady-state fluorescence spectroscopy and spectrally resolved confocal microscopy. The shape of the emission spectrum...

Liu, K.; Xu, S.; Xuan, W.; Ling, T.; Cao, Z.; Huang, B.; Sun, Y.; Fang, L.; Liu, Z.; Zhao, N.; Shen, W., 2007: Carbon monoxide counteracts the inhibition of seed germination and alleviates oxidative damage caused by salt stress in Oryza sativa. Although carbon monoxide (CO) has always been regarded as a toxic gas, recent reports suggested that it is one of the products of heme oxygenase (HO; EC 1.14.99.3) catalysis in animals, and could confer beneficial cytoprotection against oxidative...

Hsieh, C-Tung.; Hwu, W-Liang.; Huang, Y-Te.; Huang, A-Chu.; Wang, S-Fang.; Hu, M-Huei.; Chien, Y-Hsiu., 2008: Early detection of glutaric aciduria type I by newborn screening in Taiwan. Background/Purpose: Glutaric aciduria type 1 (GA1) is an inborn error of lysine and tryptophan metabolism. There is a lack of initial diagnostic signs of the disease, but late treatment often results in severe neurologic impairment. In this study,...

Schulemann, W.; Knocke, E., 1941: Zur Problem der exoerythrocytaren Entwicklungsformen von Plasmodium gallinaceum. I. Archiv fuer Experimentelle Pathologie und Pharmakologie (Germany): 197: 227-239

Leitch, J.L.; Liebig, C.S.; Haley, T.J., 1954: The use of the rat's isolated seminal vesicle for the assay of sympatholytic drugs. British Journal of Pharmacology and ChemoTherapy 9(2): 236-239

Schneider B.; Parker S., 1990: Does stimulus context affect loudness or only loudness judgments. Marks (1988) reported that when equal-loudness matches were inferred from magnitude estimates of loudness for tones of two different frequencies, the matches were affected by changes in the stimulus intensity range at both frequencies. Marks inter...

Pruniéras, M.; Cachard-Berger, D.; Durepaire, R.M.; Grupper, C., 1971: Study on hematoderma. I. Electronic microscopy. Annales de Dermatologie et de Syphiligraphie 98(3): 316-321

Cauhepe, F.Eux, C.Utand, B.Uvet, 1955: The morphogenetic role of neuromuscular behavior. Revue de Stomatologie 56(7): 535-538