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Utilization of amino acids, peptides, and protein by cultures of embryonic heart

, : Utilization of amino acids, peptides, and protein by cultures of embryonic heart. Journal of the National Cancer Institute 14(3): 519-525

Twenty-five percent chick-embryo extract, used as the nutrient medium for the cultivation of chick heart fibroblasts, contained approx. 100 mg. of nonprotein amino acids/100 ml. Almost 25% of these were in conjugated form. During cultivation both free and conjugated amino acids, as well as protein, were removed from the medium. When labeled embryo extract containing C14-amino acids, peptides, and protein was employed, it was possible to equate C14 losses from these fractions with corresponding gains by the heart cultures. Partial proteolytic digests and complete acid hydro-lysates of Cl4-labeled protein were rapidly incorporated into tissue cultures, and at approx. equal rates, when added to the nutrient medium.


PMID: 13233810

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