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Effects of mutagenic treatment by ethidium bromide on cellular and mitochondrial phenotype

, : Effects of mutagenic treatment by ethidium bromide on cellular and mitochondrial phenotype. Archives of Biochemistry and Biophysics 148(1): 115-129

The phenotypic consequences of quantitative mutagenesis by ethidium bromide (EB) have been investigated. When starved cells (2 × 107/ml in phosphate buffer) of Saccharomyces cerevisiae are treated with 20 g EB/ml for 150 min, they generate respiration deficient cytoplasmic petite (p) clones to the extent of greater than 98%. Phenotypically such a population of EB-induced cells (EBIC) growing in the absence of the mutagen is initially respiration sufficient but loses its capability of growth on a nonfermentable carbon source (e.g., lactate) within 2–3 mass doublings. In contrast EBIC are capable of continued and sustained growth on galactose, a fermentable but weakly repressing carbon source. Under all these conditions the initial phenotypic lesion appears to be confined to the inner mitochondrial membrane and is manifested by an inability of elaborating cytochrome oxidase activity and cytochromes a·a3+. These results are explained in terms of an EB-induced modification of mitochondrial DNA which makes impossible the continued transcription of a gene product(s) essential for the functions just described.


PMID: 4333686

DOI: 10.1016/0003-9861(72)90122-1

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