+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mechanism of bilirubin toxicity on tissue culture cells: factors that affect toxicity, reversibility by albumin, and comparison with other respiratory poisons and surfactants

, : Mechanism of bilirubin toxicity on tissue culture cells: factors that affect toxicity, reversibility by albumin, and comparison with other respiratory poisons and surfactants. Biochemical Medicine 5(1): 1-16

Various factors that influence the cytotoxic effects of bilirubin, a known electron-transport poison, on mammalian cells in culture have been studied. The cellular uptake of free bilirubin is rapid, being complete in 10 minutes or less following its addition to a cell suspension. Increasing bilirubin concentration increased cell death; there is a lag phase at all concentrations tested, inversely proportional to the concentration of the bile pigment seen with this effect on viability. Cytotoxicity increased at pH 7.6 and below. Albumin-bound bilirubin was nontoxic to tissue culture cells. Albumin added to bilirubin-treated cells resulted in extraction of significant quantities of the cellular-bound bilirubin. However, even when the albumin was added as rapidly as 1 minute after the bilirubin to cells there was still some loss in cell viability. Uncouplers of oxidative phosphorylation and inhibitors of NADH oxidase activity, unlike bilirubin, neither decreased cell viability nor brought about the loss of soluble proteins from cells. Bilirubin more closely resembled agents such as deoxycholate that affect cell membranes in bringing about the loss of cellular proteins. The suggestion is made that the electron-transport lesion induced by bilirubin may not in itself bring about cell death, but that the primary lethal lesion may be on multiple cell membrane systems, perhaps brought about by some alteration of membrane lipids.


PMID: 5167173

DOI: 10.1016/0006-2944(71)90069-x

Other references

Ranjan, R.; Khazen, G.; Gambazzi, L.; Ramaswamy, S.; Hill, S.L.; Schürmann, F.; Markram, H., 2012: Channelpedia: an integrative and interactive database for ion channels. Ion channels are membrane proteins that selectively conduct ions across the cell membrane. The flux of ions through ion channels drives electrical and biochemical processes in cells and plays a critical role in shaping the electrical properties of...

Cohen, J., 1995: High turnover of HIV in blood revealed by new studies. Science 267(5195): 179-179

Schroeder, W.; Treumann, F.; Rathscheck, W.; Müller, R., 1976: Muscle-pO2 in trained and untrained non-anesthetized guinea pigs and in men. pO2-measurements were performed by micro-Pt-electrodes a) in the gastrocnemius muscles of physically trained and untrained non-anesthetized guinea pigs, and b) in the tibialis anterior muscles of physically trained and untrained men, and of patien...

Jensen, R.D.; Copeland, S.; Domas, S.; Hampton, R.; Hoyme, K.; Jump, M.; Rekik, I.; Schwartz, S.; Vasserman, E., 2017: A Roundtable Discussion: Thawing Out Healthcare Technology's 'Special Snowflake' Cybersecurity Challenges. Biomedical Instrumentation & Technology 51(S6): 10-16

Beals, S.P.; Joganic, E.F., 2005: Form and function in craniofacial deformities. The interplay of form and function is recognized throughout nature. Whether at the cellular level or visible form, physiological function will not be optimal if not supported by ideal morphology. This principle could not be more true than in the r...

Barreiro Daviña, A., 1990: Conservative alternative in pulpotomies of temporary molars. Revista de Actualidad Odontoestomatologica Espanola 50(397): 45-47

Hardcastle J.; Hardcastle P.T.; Kelleher D.K.; Fondacaro J.D., 1986: The effect of auranofin on the colonic transport of sodium and fluid in the rat. Auranofin in the mucosal fluid caused a dose-dependent inhibition of fluid and Na+ absorption by everted sacs of rat colon. Serosal auranofin was without effect. (Na+ + K+) ATPase activity of homogenates of mucosal scrapes of rat colon was inhibit...

Mramba, L.; Nassir, F.Ahmed.; Ondieki, C.; Kimanga, D., 2010: Reasons for low utilization of a maternity waiting home in rural Kenya. Concept learning is challenging in part because the meanings of many concepts depend on their relationships to other concepts. Learning these concepts in isolation can be difficult, but we present a model that discovers entire systems of related c...

Rubens, I.P.; Iurika, E.V.; Seleznev, I.V., 1992: Indicators of the lithogenic properties of the bile: methods of their evaluation, clinical availability and informative value. Duodenal bile taken by gastroduodenal intubation of patients with chronic calculous cholecystitis with intact gallbladder performance was investigated as was the bile taken in those with chronic acalculous cholecystitis or in those without clinica...

Honarparvar, N.; Khanjani, M.; Forghani, S.H.R.; Talebi, A.A., 2012: Effect of Temperature on Development and Fecundity of the Brown Mite, Bryobia rubrioculus Scheuten Acari Tetranychidae. The brown mite, <i>Bryobia rubrioculus</i> Scheuten thrives on sweet cherry, sour cherry and plum orchards of Hamedan in the western region of Iran. Development and fecundity of this mite was studied on sweet cherry at nine constant te...